Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42028
Seitanidou T, et al., Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain. Mech Dev. 1997 Jul;65(1-2):31-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5436418
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Gene symbol:
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Fst
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Gene name:
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follistatin
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Probe:
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follistatin probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4E:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more  )
Note: 7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
Specimen
4E:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4E
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Expression was detected in rhombomere 2 and 4.
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Specimen
4F:
embryonic day 8.0; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
Specimen
4F:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4F
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Expression was detected in a continuous rhombomere 2,3,4 domain extending caudally to the rhombomere 4/5 boundary. The staining hid Egr2 lacZ staining in rhombomere 3 (see assay MGI:5436312).
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Specimen
4G:
embryonic day 8.0
(more )
Note: 6 somites.
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4G
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Expression was detected in rhombomere 2 and 4.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
4H
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Expression was detected in all cells within the rhombomere 2,3,4 domain, but not in the prospective rhombomere 5 territory.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: rhombomere 2 |
Present |
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| TS13: rhombomere 4 |
Present |
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| TS13: rhombomere 5 |
Absent |
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| TS13: rhombomere |
Present |
Regionally restricted |
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Expression was maintained in rhombomere 2 and 4. At 12 somites, rhombomere 3 has almost disappeared in the mutant embryos. Rhombomere 5 remained negative, and a low signal appeared in rhombomere 6.
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Analysis Tools