Probe:	Raldh2 probe6
Probe preparation:	Antisense

Structure	Level	Pattern	Image	Note
TS18: duodenum caudal part	Strong	Non-Uniform	2A	Robustly expressed in the proximal intestine, with diminishing intensity distally.
TS18: duodenum rostral part	Strong	Non-Uniform	2A	Robustly expressed in the proximal intestine, with diminishing intensity distally.
TS18: midgut loop	Absent		2A	No expression in the early cecum.
TS18: hindgut	Absent		2A	No expression in the early colon.

Structure	Level	Pattern	Image	Note
TS19: duodenum caudal part mesoduodenum	Strong	Regionally restricted	2B	Expression was limited to the mesoderm, with equal intensity both in the proximal and distal duodenum.
TS19: duodenum rostral part mesenchyme	Strong	Regionally restricted	2B	Expression was limited to the mesoderm, with equal intensity both in the proximal and distal duodenum.

Structure	Level	Pattern	Image	Note
TS19: duodenum caudal part	Strong		3A	Robust expression both in the proximal and distal duodenum.
TS19: duodenum rostral part	Strong		3A	Robust expression both in the proximal and distal duodenum.

Structure	Level	Pattern	Image	Note
TS19: duodenum rostral part	Present		3B	Reduced expression in the proximal duodenum.
TS19: duodenum caudal part	Strong		3B	Robust expression in the distal duodenum.

Structure	Level	Pattern	Image	Note
TS20: duodenum caudal part	Strong		4A	Robust expression both in the proximal and distal duodenum.
TS20: duodenum rostral part	Strong		4A	Robust expression both in the proximal and distal duodenum.

Structure	Level	Pattern	Image	Note
TS20: duodenum caudal part	Present	Regionally restricted	4B	Reduced expression in the very focal within the proximal duodenum where the atresia was forming. Reduced overall expression in the proximal intestine, compared with the wild type.
TS20: duodenum rostral part	Present	Regionally restricted	4B	Reduced expression in the very focal within the proximal duodenum where the atresia was forming. Reduced overall expression in the proximal intestine, compared with the wild type.

Structure	Level	Pattern	Image	Note
TS20: duodenum caudal part	Present	Regionally restricted	4C	Reduced expression in both the proximal and distal duodenum, compared with the wild type. Expression was uniform when no atresia formed. Expression was restricted to the mesenteric side. Little or no expression within the antimesenteric mesoderm.
TS20: duodenum rostral part	Present	Regionally restricted	4C	Reduced expression in both the proximal and distal duodenum, compared with the wild type. Expression was uniform when no atresia formed. Expression was restricted to the mesenteric side. Little or no expression within the antimesenteric mesoderm.