Probe:	Lepre1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		S1_d
TS17: notochord	Present		S1_d

Structure	Level	Pattern	Image	Note
TS15: notochord	Weak		3a
TS15: otocyst	Weak		3a	There is weak expression in the otic capsule.
TS15: head	Weak		3a	There is weak expression in craniofacial tissues.
TS15: forelimb bud	Absent		3a	There is little to no expression.
TS15: trunk paraxial mesenchyme	Absent		3a	There is little to no expression.

Structure	Level	Pattern	Image	Note
TS15: notochord	Weak		3b
TS15: otocyst	Weak		3b	There is weak expression in the otic capsule.
TS15: head	Weak		3b	There is weak expression in craniofacial tissues.
TS15: forelimb bud	Absent		3b	There is little to no expression.
TS15: trunk paraxial mesenchyme	Absent		3b	There is little to no expression.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		3d	Expression is present in craniofacial/branchial arch tissues.
TS17: head	Present		3d	Expression is present in craniofacial/branchial arch tissues.
TS17: otocyst	Present		3d	Expression is present in the otic capsule.
TS17: paraxial mesenchyme	Present		3d
TS17: hindlimb bud	Present		3d
TS17: forelimb bud	Absent		3d

Structure	Level	Pattern	Image	Note
TS17: notochord	Present		3e , 3f

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		3g

Structure	Level	Pattern	Image	Note
TS19: head	Present		3i	Expression is waning in craniofacial tissues.
TS19: inner ear	Present		3i	Expression is present in the otic capsule.
TS19: hindlimb bud	Present		3i	Expression is waning.
TS19: paraxial mesenchyme	Present		3i	Expression is waning.

Structure	Level	Pattern	Image	Note
TS19: notochord	Absent		3k , 3j	Expression is absent from domains adjacent and/or anterior to the forelimb.

Structure	Level	Pattern	Image	Note
TS19: notochord	Present	Regionally restricted	3m , 3l	Expression is present in rostral domains.

Structure	Level	Pattern	Image	Note
TS17: notochord	Present		6a' Leprecan E10.5	Inset shows higher magnification of staining in the notochord.

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		6a' Leprecan E11.5

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		6a' Leprecan E12.5

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Regionally restricted	6a' Leprecan E13.5	Expression is present in the notochord.
TS21: vertebral cartilage condensation	Present	Regionally restricted	6a' Leprecan E13.5	Expression is present in the neural arches and laminae of the developing vertebrae.

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present		6b'a
TS20: humerus pre-cartilage condensation	Present		6b'a

Structure	Level	Pattern	Image	Note
TS20: femur pre-cartilage condensation	Present		6b'b
TS20: hindlimb pre-cartilage condensation	Present		6b'b

Structure	Level	Pattern	Image	Note
TS20: vertebral pre-cartilage condensation	Present		6b'c	Expression is present in the vertebral mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: forelimb pre-cartilage condensation	Present		6b'd
TS21: humerus cartilage condensation	Present		6b'd

Structure	Level	Pattern	Image	Note
TS21: femur cartilage condensation	Present	Regionally restricted	6b'e	Expression is present in the proximal femur.
TS21: pelvic girdle skeleton	Present		6b'e

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	6b'f	Expression is present in the centrum, the transverse processes, and laminae.

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	6b'g	Expression is present in the neural arches.