Probe:	MnSod cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: amnion	Present		3A
TS11: chorion	Present		3A
TS11: ectoplacental cone	Present		3A
TS11: embryo ectoderm	Present		3A
TS11: neural fold	Present		3A

Structure	Level	Pattern	Image	Note
TS13: ectoplacental cone	Present		3B
TS13: gut	Present		3B
TS13: heart	Present		3B
TS13: neural fold	Present		3B
TS13: extraembryonic component	Present	Regionally restricted	3B	Expression was detected in the outer extraembryonic membranes.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		3C
TS15: telencephalon	Present		3C
TS15: future hindbrain	Present	Regionally restricted	3C	Expression was detected in the myelencephalon.
TS15: 1st branchial arch	Present		3C
TS15: forelimb bud	Present		3C
TS15: future spinal cord	Present		3C
TS15: gut	Present		3C
TS15: otocyst	Present		3C
TS15: olfactory placode	Present	Regionally restricted	3C	Expression was detected in the olfactory pit.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3D
TS17: future spinal cord	Present		3D
TS17: heart	Present		3D
TS17: hindlimb bud	Present		3D
TS17: midbrain	Present		3D
TS17: primitive ventricle	Present		3D
TS17: telencephalon	Present		3D

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		3E
TS19: future spinal cord	Present		3E
TS19: hindlimb bud	Present		3E
TS19: midbrain	Present		3E
TS19: telencephalon	Present		3E

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present		3F
TS20: hindlimb	Present		3F
TS20: midbrain	Present		3F
TS20: spinal cord	Present		3F
TS20: telencephalon	Present		3F

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Moderate
TS21: choroid plexus	Strong
TS21: cerebellum	Moderate
TS21: remnant of Rathke's pouch	Strong
TS21: central nervous system ganglion	Very strong
TS21: lobar bronchus epithelium	Strong
TS21: lung parenchyma	Strong
TS21: olfactory epithelium	Strong
TS21: spinal cord	Strong
TS21: cardiac muscle tissue	Moderate
TS21: blood	Very strong	Regionally restricted		Expression was very strong in blood cells.
TS21: blood vessel	Strong
TS21: intestine epithelium	Strong
TS21: intestine muscularis	Moderate
TS21: liver	Strong	Regionally restricted		Expression was strong in hepatocytes and very strong in hematopoietic cells.
TS21: pancreas	Present	Regionally restricted		Expression was strong in acinus and moderate in islet cells.
TS21: renal corpuscle	Moderate
TS21: renal tubule	Moderate
TS21: submandibular gland primordium	Moderate
TS21: gland	Strong	Regionally restricted		Expression was strong in adrenal gland medulla and cortex.
TS21: thymus primordium	Moderate
TS21: skeleton	Strong			Expression was strong in bone.
TS21: skeletal muscle	Weak
TS21: skin	Weak

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Strong
TS22: cerebral cortex	Strong
TS22: choroid plexus	Strong
TS22: remnant of Rathke's pouch	Strong
TS22: central nervous system ganglion	Very strong
TS22: inner ear epithelium	Strong
TS22: lobar bronchus epithelium	Strong
TS22: lung parenchyma	Strong
TS22: olfactory epithelium	Strong
TS22: spinal cord	Strong
TS22: cardiac muscle tissue	Moderate
TS22: blood	Very strong	Regionally restricted		Expression was very strong in blood cells.
TS22: blood vessel	Strong
TS22: intestine epithelium	Strong
TS22: intestine muscularis	Moderate
TS22: liver	Very strong	Regionally restricted		Expression was very strong in hepatocyte and hematopoietic cells.
TS22: pancreas	Strong	Regionally restricted		Expression was strong in acinus and islet cells.
TS22: renal corpuscle	Moderate
TS22: submandibular gland primordium	Moderate
TS22: adrenal cortex	Strong
TS22: adrenal medulla	Strong
TS22: renal tubule	Strong
TS22: skeleton	Strong
TS22: thymus primordium	Strong
TS22: skeletal muscle	Moderate
TS22: skin	Moderate

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Strong
TS23: cerebral cortex	Strong
TS23: choroid plexus	Strong
TS23: central nervous system ganglion	Very strong
TS23: inner ear epithelium	Very strong
TS23: olfactory epithelium	Very strong
TS23: spinal cord	Very strong
TS23: cardiac muscle tissue	Strong
TS23: lobar bronchus epithelium	Strong
TS23: lung parenchyma	Strong
TS23: blood	Very strong	Regionally restricted		Expression was very strong in blood cells.
TS23: blood vessel	Strong
TS23: intestine epithelium	Strong
TS23: intestine muscularis	Moderate
TS23: liver	Very strong	Regionally restricted		Expression was very strong in hepatocyte and hematopoietic cells.
TS23: pancreas	Present	Regionally restricted		Expression was moderate in acinus and strong in islet cells.
TS23: submandibular gland primordium	Strong
TS23: renal corpuscle	Moderate
TS23: renal tubule	Strong
TS23: detrusor muscle of bladder	Moderate
TS23: urothelium of bladder	Moderate
TS23: adrenal medulla	Strong
TS23: adrenal cortex	Moderate
TS23: thymus primordium	Strong
TS23: skeletal muscle	Moderate
TS23: skeleton	Moderate
TS23: skin	Present	Regionally restricted		Expression was moderate in skin and strong in brown adipose tissue.

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Strong
TS24: cerebral cortex	Strong
TS24: choroid plexus	Strong
TS24: remnant of Rathke's pouch	Moderate
TS24: central nervous system ganglion	Very strong
TS24: inner ear epithelium	Very strong
TS24: olfactory epithelium	Very strong
TS24: spinal cord	Very strong
TS24: lobar bronchus epithelium	Strong
TS24: lung parenchyma	Strong
TS24: cardiac muscle tissue	Moderate
TS24: blood	Very strong	Regionally restricted		Expression was very strong in blood cells.
TS24: blood vessel	Strong
TS24: intestine epithelium	Strong
TS24: intestine muscularis	Strong
TS24: liver	Present	Regionally restricted		Expression was very strong in hepatocyte and moderate in hematopoietic cells.
TS24: pancreas	Moderate	Regionally restricted		Expression was moderate in acinus and islet cells.
TS24: submandibular gland	Strong
TS24: renal corpuscle	Moderate
TS24: detrusor muscle of bladder	Strong
TS24: renal tubule	Strong
TS24: thymus	Strong
TS24: urothelium of bladder	Strong
TS24: skeleton	Moderate
TS24: skin	Very strong
TS24: skeletal muscle	Strong
TS24: brown fat	Very strong

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Strong
TS25: cerebral cortex	Strong
TS25: choroid plexus	Strong
TS25: remnant of Rathke's pouch	Moderate
TS25: central nervous system ganglion	Very strong
TS25: spinal cord	Very strong
TS25: inner ear epithelium	Strong
TS25: lobar bronchus epithelium	Strong
TS25: lung parenchyma	Strong
TS25: olfactory epithelium	Strong
TS25: cardiac muscle tissue	Moderate
TS25: blood	Strong	Regionally restricted		Expression was strong in blood cells.
TS25: blood vessel	Strong
TS25: intestine epithelium	Strong
TS25: intestine muscularis	Strong
TS25: liver	Present	Regionally restricted		Expression was strong in hepatocyte and moderate in hematopoietic cells.
TS25: pancreas	Present	Regionally restricted		Expression was strong in acinus and moderate in islet cells.
TS25: submandibular gland	Strong
TS25: renal corpuscle	Moderate
TS25: adrenal medulla	Strong
TS25: detrusor muscle of bladder	Strong
TS25: renal tubule	Strong
TS25: urothelium of bladder	Strong
TS25: adrenal cortex	Moderate
TS25: thymus	Strong
TS25: skeleton	Moderate
TS25: skin	Very strong
TS25: skeletal muscle	Moderate
TS25: brown fat	Strong

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong
TS26: cerebellum	Moderate
TS26: choroid plexus	Moderate
TS26: remnant of Rathke's pouch	Moderate
TS26: central nervous system ganglion	Very strong
TS26: spinal cord	Very strong
TS26: inner ear epithelium	Strong
TS26: lobar bronchus epithelium	Strong
TS26: lung parenchyma	Strong
TS26: olfactory epithelium	Strong
TS26: cardiac muscle tissue	Weak
TS26: blood	Strong	Regionally restricted		Expression was strong in blood cells.
TS26: blood vessel	Strong
TS26: intestine epithelium	Strong
TS26: intestine muscularis	Moderate
TS26: liver	Present	Regionally restricted		Expression was strong in hepatocyte and weak in hematopoietic cells.
TS26: pancreas	Moderate	Regionally restricted		Expression was moderate in acinus and islet cells.
TS26: renal corpuscle	Strong
TS26: renal tubule	Strong
TS26: submandibular gland	Strong
TS26: adrenal medulla	Moderate
TS26: detrusor muscle of bladder	Moderate
TS26: adrenal cortex	Weak
TS26: thymus	Strong
TS26: skeleton	Moderate
TS26: skin	Very strong
TS26: skeletal muscle	Moderate
TS26: brown fat	Strong

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Regionally restricted	4A	Expression was detected at high levels in the external layers of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS26: cerebellum lateral part marginal layer	Present		4B

Structure	Level	Pattern	Image	Note
TS25: central nervous system ganglion	Very strong	Regionally restricted	4C	Expression was very strong in neurons.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Very strong	Regionally restricted	4D	Expression was very strong in neurons.

Structure	Level	Pattern	Image	Note
TS23: olfactory epithelium	Strong		4E

Structure	Level	Pattern	Image	Note
TS24: inner ear epithelium	Strong		4F

Structure	Level	Pattern	Image	Note
TS22: lobar bronchus epithelium	Present		4G
TS22: lung parenchyma	Present		4G

Structure	Level	Pattern	Image	Note
TS25: lobar bronchus epithelium	Present		4H
TS25: lung parenchyma	Present		4H

Structure	Level	Pattern	Image	Note
TS23: blood	Present	Regionally restricted	4I	Expression was high in blood cells.

Structure	Level	Pattern	Image	Note
TS25: ventricle cardiac muscle	Moderate		4J

Structure	Level	Pattern	Image	Note
TS25: stomach	Present	Regionally restricted	4K	Expression was high in the luminal epithelium and muscular layer.
TS25: stomach epithelium	Present		4K

Structure	Level	Pattern	Image	Note
TS25: intestine epithelium	Present		4L
TS25: intestine muscularis	Present		4L

Structure	Level	Pattern	Image	Note
TS22: liver	Very strong	Regionally restricted	4M	Expression was very strong in hepatocytes and hematopoietic cells.

Structure	Level	Pattern	Image	Note
TS25: liver	Very strong	Regionally restricted	4N	Expression was very strong in hepatocytes.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Strong	Regionally restricted	4O	Expression was strong in pancreatic acini.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Strong	Regionally restricted	4P	Expression was strong in pancreatic islets.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Strong		4Q

Structure	Level	Pattern	Image	Note
TS26: renal tubule	Strong		4R

Structure	Level	Pattern	Image	Note
TS25: detrusor muscle of bladder	Present		4S
TS25: urothelium of bladder	Present		4S

Structure	Level	Pattern	Image	Note
TS25: skeleton	Moderate	Regionally restricted	4T	Expression was moderate in osteogenic tissue.

Structure	Level	Pattern	Image	Note
TS22: skin	Moderate		4U

Structure	Level	Pattern	Image	Note
TS26: skin	Strong		4V

Structure	Level	Pattern	Image	Note
TS25: brown fat	Present		4W