Gene Expression Data
Assay Details
Assay
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Reference:
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J:106092
Tissir F, et al., Expression of planar cell polarity genes during development of the mouse CNS. Eur J Neurosci. 2006 Feb;23(3):597-607
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4946667
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Gene symbol:
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Vangl2
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Gene name:
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VANGL planar cell polarity 2
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Probe:
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Vangl2 cDNA3
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Probe preparation:
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Antisense labelled with P33 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1G:
embryonic day 10.5
(more )
Specimen
1G:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: nervous system |
Very strong |
Widespread |
1G
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Expression was very strong in all neural structures, particularly in ventricular zones.
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TS17: limb |
Moderate |
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1G
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TS17: tail |
Moderate |
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1G
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Specimen
2Q:
embryonic day 12.5
(more )
Specimen
2Q:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
2R:
embryonic day 12.5
(more )
Specimen
2R:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
2S & 2T:
embryonic day 12.5
(more )
Note: Fig. 2T is a higher magnification image of the boxed region if Fig. 2S.
Specimen
2S & 2T:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Fig. 2T is a higher magnification image of the boxed region if Fig. 2S.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: brain |
Present |
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2S
,
2T
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Expression was evenly distributed, somewhat higher in ventricular zone and postmitotic differentiation fields.
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TS20: brain ventricular layer |
Present |
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2S
,
2T
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Expression was evenly distributed, somewhat higher in ventricular zone and postmitotic differentiation fields.
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Specimen
3F:
embryonic day 14.5
(more )
Specimen
3F:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
Table 2:
embryonic day 17.5
(more )
Specimen
Table 2:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: brainstem |
Ambiguous |
Regionally restricted |
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Expression was present in the cochleovestibular nuclei and very low or undetectable in the reticular formation.
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TS25: cerebellum |
Ambiguous |
Regionally restricted |
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Expression was very low or undetectable in the external granule layer.
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TS25: midbrain ventricular layer |
Strong |
|
|
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TS25: hypothalamus |
Ambiguous |
|
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Expression was very low or undetectable.
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TS25: midbrain tegmentum |
Ambiguous |
|
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Expression was very low or undetectable.
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TS25: tectum |
Ambiguous |
|
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Expression was very low or undetectable.
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TS25: diencephalon ventricular layer |
Present |
|
|
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TS25: thalamus |
Ambiguous |
Regionally restricted |
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Expression was very low or undetectable in dorsal and ventral thalamus.
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TS25: retina |
Strong |
Regionally restricted |
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Expression was high in the ventricular zone and ganglion cells.
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TS25: cortical plate |
Present |
Regionally restricted |
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Expression was high in the cortical plate ventricular zone/subventricular zone and present in the cortical plate proper.
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TS25: corpus striatum |
Present |
Regionally restricted |
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Expression was high in the striatum ventricular zone/subventricular zone and present in the striatum.
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TS25: main olfactory bulb |
Present |
Regionally restricted |
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Expression was detected in the olfactory bulb and mitral cells.
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Specimen
4F2:
embryonic day 17.5
(more )
Specimen
4F2:
(close )
Genetic Background:
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CD-1
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: brain |
Present |
Regionally restricted |
4F2
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Expression was more intense in ventricular zone than in differentiating neuronal fields.
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TS25: brain ventricular layer |
Present |
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4F2
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Expression was more intense in ventricular zone than in differentiating neuronal fields.
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Specimen
4F3:
embryonic day 17.5
(more )
Specimen
4F3:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cortical plate |
Moderate |
Regionally restricted |
4F3
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Expression was detected in the medial and dorsal sectors of the cortical plate.
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TS25: brain |
Present |
Regionally restricted |
4F3
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Expression was more intense in ventricular zone than in differentiating neuronal fields.
|
TS25: brain ventricular layer |
Present |
|
4F3
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Expression was more intense in ventricular zone than in differentiating neuronal fields.
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Specimen
4F4:
embryonic day 17.5
(more )
Specimen
4F4:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5J:
postnatal day 0
(more )
Specimen
5J:
(close )
Genetic Background:
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CD-1
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Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Table 3:
postnatal day 10
(more )
Specimen
Table 3:
(close )
Genetic Background:
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CD-1
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Specimen
6P:
postnatal day 10
(more )
Specimen
6P:
(close )
Genetic Background:
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CD-1
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Age:
|
postnatal day 10
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6Q:
postnatal day 10
(more )
Specimen
6Q:
(close )
Genetic Background:
|
CD-1
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Age:
|
postnatal day 10
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Specimen
6R:
postnatal day 10
(more )
Specimen
6R:
(close )
Genetic Background:
|
CD-1
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Age:
|
postnatal day 10
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7F:
postnatal day 21
(more )
Specimen
7F:
(close )
Genetic Background:
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CD-1
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Age:
|
postnatal day 21
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: dentate gyrus |
Present |
|
7F
|
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TS28: brain |
Present |
Regionally restricted |
7F
|
Expression was diffuse with significant labelling in the residual ventricular zone around the lateral ventricles.
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