Reporter:	lacZ
	Direct Detection
Assay notes:	Fixative was 2% paraformaldehyde and 0.2% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present		2A	Expression is in neural tissues.
TS20: spinal cord	Very strong		2A
TS20: telencephalon	Very strong	Regionally restricted	2A	Expression is in the olfactory lobe.

Structure	Level	Pattern	Image	Note
TS11: future brain neural fold	Weak		3A

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present		3B
TS13: rhombomere 2	Strong		3B
TS13: rhombomere 3	Strong		3B

Structure	Level	Pattern	Image	Note
TS15: rhombomere	Present		3C
TS15: future spinal cord	Present	Ubiquitous	3C

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Strong		3D
TS17: cranial ganglion	Very strong		3D	Intense segmental expression in each ganglion and in the neurites of each one.
TS17: spinal component of peripheral nervous system	Very strong		3D	Intense segmental expression in each ganglion and in the neurites of each one.
TS17: forebrain	Weak		3D
TS17: midbrain	Weak		3D

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural fold	Weak	Regionally restricted	4A	Expression is at the luminal surface of the neuroectoderm.
TS13: notochord	Present		4A

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	4B	Expression is at the ventricular surface of neuroepithelial cells. Faint expression at the subpial surface of the basal plate.
TS15: trunk mesenchyme derived from neural crest	Weak	Regionally restricted	4B	Expression is along somites.

Structure	Level	Pattern	Image	Note
TS19: neural tube marginal layer	Very strong		4D , 4C
TS19: neural tube mantle layer	Present		4C , 4D
TS19: dorsal root ganglion	Present		4C
TS19: myotome	Absent		4D , 4C
TS19: neural tube ventricular layer	Absent		4D , 4C
TS19: sclerotome	Absent		4D , 4C

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		5A
TS15: rhombomere	Present		5A

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		5B , 5C
TS15: rhombomere 1	Present		5B , 5C
TS15: rhombomere 2	Present		5B , 5C
TS15: rhombomere 4	Present		5B , 5C
TS15: rhombomere 5	Present		5B , 5C
TS15: rhombomere 3	Weak		5B , 5C

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural fold	Weak	Regionally restricted	5D	Expression is at the luminal surface of the neuroectoderm and is absent at the lateral edges of the neural groove.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	6B , 6A	Expression is absent in the subventricular zones, expression was found in the outgrowth of neurites, neural fibers.
TS20: medulla oblongata	Present	Regionally restricted	6B , 6A	Expression is absent in the subventricular zones, expression was found in the outgrowth of neurites, neural fibers.
TS20: midbrain	Present	Regionally restricted	6B , 6A	Expression is absent in the subventricular zone, present in subpial surfaces, neural fibers.
TS20: telencephalon	Present	Regionally restricted	6B , 6A	Expression is absent in the subventricular zone, present in subpial surfaces, neural fibers.
TS20: metencephalon	Present	Regionally restricted	6B , 6A	Expression was found in the outgrowth of neurites, neural fibers.

Structure	Level	Pattern	Image	Note
TS21: cartilage	Present	Regionally restricted		Expression is in the perichondrium.
TS21: liver	Present
TS21: nervous system	Present
TS21: thymus primordium	Absent