Probe:	Arnt2 cDNA3
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: future brain	Strong		3a
TS15: neural tube	Strong		3a

Structure	Level	Pattern	Image	Note
TS17: brain	Strong		3b
TS17: neural tube	Strong		3b

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Strong		3c	The signal was strong in the neocortical neuroepithelium and the subventricular zone, weak in the differentiating field.
TS25: neocortex	Strong		3c	The signal was strong in the neocortical neuroepithelium and the subventricular zone, weak in the differentiating field.
TS25: cortical plate	Present	Regionally restricted	3c	Expression is in the cortical plate in the infra- and supragranular parts.
TS25: retina inner nuclear layer	Strong		3c

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Very strong	Ubiquitous	3d
TS19: midbrain	Very strong	Regionally restricted	3d	Expression is in the tectal and tegmental neuroepithelium, the tegmental differentiating field, and the differentiating fields of the superior and inferior colliculi.
TS19: branchial arch	Strong		3d
TS19: cerebellum primordium	Strong		3d
TS19: nasal process	Strong		3d
TS19: limb	Moderate		3d
TS19: intestine	Weak		3d
TS19: heart	Absent		3d
TS19: liver	Absent		3d

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Very strong		3e
TS21: hypothalamus	Very strong		3e
TS21: medulla oblongata	Very strong		3e
TS21: pituitary gland	Very strong		3e
TS21: spinal cord	Very strong		3e
TS21: thalamus	Very strong		3e
TS21: midbrain	Very strong	Regionally restricted	3e	Expression is in the tectal and tegmental neuroepithelium, the tegmental differentiating field, and the differentiating fields of the superior and inferior colliculi.
TS21: eye	Strong		3e
TS21: genital tubercle	Moderate		3e
TS21: limb	Moderate		3e
TS21: tongue	Moderate		3e
TS21: intestine	Weak		3e
TS21: lung epithelium	Weak		3e
TS21: lung mesenchyme	Weak		3e
TS21: vibrissa follicle	Weak		3e
TS21: vertebral cartilage condensation	Weak	Regionally restricted	3e	backbone
TS21: liver	Absent		3e

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Strong		3f
TS21: limb	Moderate		3f
TS21: lung epithelium	Weak		3f
TS21: lung mesenchyme	Weak		3f
TS21: vibrissa follicle	Weak		3f

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present		3g
TS23: ear	Present		3g
TS23: eye	Present		3g
TS23: limb	Present		3g
TS23: lung epithelium	Present		3g
TS23: lung mesenchyme	Present		3g
TS23: metanephros	Present		3g
TS23: neocortex	Present		3g
TS23: stomach	Present		3g
TS23: telencephalon septum	Present		3g
TS23: thalamus	Present		3g
TS23: tongue	Present		3g
TS23: trachea epithelium	Present		3g
TS23: trachea mesenchyme	Present		3g

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present		3h
TS23: cochlear duct epithelium	Present		3h
TS23: cochlear duct mesenchyme	Present		3h
TS23: limb	Present		3h
TS23: neural retina	Present		3h

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Very strong		3i
TS25: retina	Very strong		3i
TS25: bladder	Present		3i
TS25: esophagus	Present		3i
TS25: olfactory epithelium	Present		3i
TS25: pituitary gland	Present		3i
TS25: submandibular gland	Present		3i
TS25: thymus	Present		3i
TS25: tooth mesenchyme	Present		3i
TS25: tongue	Weak		3i
TS25: skeleton	Absent		3i	absent in backbone
TS25: liver	Absent		3i

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Very strong		3j
TS27: midbrain tegmentum	Very strong		3j
TS27: superior colliculus	Very strong		3j
TS27: tectum	Very strong		3j
TS27: basal ganglia	Strong		3j
TS27: cerebral cortex	Strong		3j
TS27: corpus striatum	Strong		3j
TS27: hippocampus	Strong		3j
TS27: hypothalamus	Strong		3j
TS27: main olfactory bulb	Strong		3j
TS27: medulla oblongata	Strong		3j
TS27: pallidum	Strong		3j
TS27: pituitary gland	Strong		3j
TS27: pons	Strong		3j
TS27: thalamus	Strong		3j
TS27: cerebellum Purkinje cell layer	Moderate		3j
TS27: cerebellum external granule cell layer	Moderate		3j
TS27: esophagus	Moderate		3j
TS27: olfactory epithelium	Moderate		3j
TS27: thymus	Moderate		3j
TS27: limb	Absent		3j
TS27: lung	Absent		3j
TS27: skeletal muscle	Absent		3j
TS27: submandibular gland	Absent		3j
TS27: tongue	Absent		3j
TS27: vibrissa follicle	Absent		3j
TS27: blood vessel	Absent		3j	Walls of large vessels.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Strong	Regionally restricted	4a	The signal was strong in the neocortical neuroepithelium and the subventricular zone, weak in the differentiating field.
TS25: neocortex	Strong	Regionally restricted	4a	The signal was strong in the neocortical neuroepithelium and the subventricular zone, weak in the differentiating field.
TS25: cortical plate	Strong	Regionally restricted	4a	Expression is in the cortical plate in the infra- and supragranular parts.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Strong		4b	The signal was strong in the neocortical neuroepithelium and the subventricular zone, weak in the differentiating field.
TS25: neocortex	Strong		4b	The signal was strong in the neocortical neuroepithelium and the subventricular zone, weak in the differentiating field.
TS25: cortical plate	Strong	Regionally restricted	4b	Expression is in the cortical plate in the infra- and supragranular parts.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong		4c

Structure	Level	Pattern	Image	Note
TS25: main olfactory bulb	Present		4d , 4e	Expression is moderate in neuroepithelium, subventricular zone, and differentiating field and very strong in mitral cell layer.

Structure	Level	Pattern	Image	Note
TS23: pallidum	Strong		4f

Structure	Level	Pattern	Image	Note
TS25: tongue muscle	Weak		4g
TS25: tongue epithelium	Absent		4g

Structure	Level	Pattern	Image	Note
TS25: submandibular gland epithelium	Weak		4h

Structure	Level	Pattern	Image	Note
TS27: intestine wall	Weak		4i
TS27: intestine smooth muscle	Present		4i

Structure	Level	Pattern	Image	Note
TS25: dental sac	Moderate		4j	Expression is in the dental follicle of the lower jaw incisor.

Structure	Level	Pattern	Image	Note
TS25: epidermis	Moderate		4k	the most superficial layer of epidermis

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present		4l
TS25: cochlear duct mesenchyme	Present		4l

Structure	Level	Pattern	Image	Note
TS21: gland	Present		4m	In adrenal gland signal is located in the medulla, whereas the signal in cortex does not exceed the background level.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Very strong		4n

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Strong		4n inset

Structure	Level	Pattern	Image	Note
TS15: neural tube	Very strong

Structure	Level	Pattern	Image	Note
TS17: neural tube	Very strong

Structure	Level	Pattern	Image	Note
TS19: neural tube	Strong
TS19: brain	Very strong
TS19: cerebellum primordium	Very strong
TS19: future spinal cord	Very strong
TS19: metencephalon basal plate	Very strong
TS19: myelencephalon	Very strong
TS19: telencephalon	Very strong	Regionally restricted		rhinencepalon, pallidum, striatum
TS19: midbrain	Very strong	Regionally restricted		tectal neuroepithelium, tegmentum neuroepithelium.
TS19: forebrain	Very strong	Regionally restricted		hippocampus
TS19: diencephalon	Very strong	Regionally restricted		thalamic and hypothalamic neuroepithelium

Structure	Level	Pattern	Image	Note
TS21: olfactory lobe	Very strong	Regionally restricted		olfactory bulb neuroepithelium, strong in subventricular zone
TS21: midbrain	Strong	Regionally restricted		Strong in the tectal neuroepithelium, tegmentum, inferior colliculus, superior colliculus.
TS21: telencephalon	Strong	Regionally restricted		Pallidal neuroepithelium and subventricular zone, septum, amygdala, very strong in rhinencephalic neuroepithelium and rhinencephalic subventricular zone.
TS21: corpus striatum	Very strong
TS21: dorsal root ganglion	Very strong
TS21: hypothalamus	Very strong
TS21: medulla oblongata	Very strong
TS21: pons	Very strong
TS21: spinal cord	Very strong
TS21: thalamus	Very strong
TS21: cerebellum	Strong
TS21: neocortex	Strong
TS21: forebrain	Weak			hippocampus, a bit stronger in subicular area

Structure	Level	Pattern	Image	Note
TS23: corpus striatum	Very strong
TS23: hypothalamus	Very strong
TS23: inferior colliculus	Very strong
TS23: main olfactory bulb	Very strong
TS23: medulla oblongata	Very strong
TS23: midbrain tegmentum	Very strong
TS23: neocortex	Very strong
TS23: pallidum	Very strong
TS23: pons	Very strong
TS23: spinal cord	Very strong
TS23: superior colliculus	Very strong
TS23: tectum	Very strong
TS23: telencephalon septum	Very strong
TS23: thalamus	Very strong
TS23: amygdala	Strong
TS23: cerebellum	Strong
TS23: dorsal root ganglion	Strong
TS23: telencephalon	Strong	Regionally restricted		rhinencephalic neuroepithelium and rhinencephalic subventricular zone.
TS23: hippocampus	Weak

Structure	Level	Pattern	Image	Note
TS25: corpus striatum	Very strong
TS25: cortical plate	Very strong
TS25: hippocampus	Very strong
TS25: hypothalamus	Very strong
TS25: inferior colliculus	Very strong
TS25: medulla oblongata	Very strong
TS25: midbrain tegmentum	Very strong
TS25: pons	Very strong
TS25: spinal cord	Very strong
TS25: superior colliculus	Very strong
TS25: tectum	Very strong
TS25: thalamus	Very strong
TS25: telencephalon	Very strong	Regionally restricted		Rhinencephalic neuroepithelium, rhinencephalic subventricular zone.
TS25: basal ganglia	Very strong	Regionally restricted		pallidum
TS25: cerebral cortex subventricular zone	Very strong
TS25: amygdala	Strong
TS25: dorsal root ganglion	Strong
TS25: adrenal gland	Present
TS25: cerebellum	Present	Regionally restricted		external germinal layer, Purkinje layer.
TS25: main olfactory bulb	Moderate			Very strong in the mitral layer.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Very strong	Regionally restricted		cortical plate
TS27: telencephalon	Very strong	Regionally restricted		rhinencepalon
TS27: hippocampus	Very strong
TS27: subiculum	Very strong
TS27: telencephalon septum	Very strong
TS27: amygdala	Strong
TS27: corpus striatum	Strong
TS27: dorsal root ganglion	Strong
TS27: hypothalamus	Strong
TS27: inferior colliculus	Strong
TS27: main olfactory bulb	Strong
TS27: midbrain tegmentum	Strong
TS27: pallidum	Strong
TS27: spinal cord	Strong
TS27: superior colliculus	Strong
TS27: tectum	Strong
TS27: thalamus	Strong
TS27: cerebellum Purkinje cell layer	Present
TS27: cerebellum external granule cell layer	Present
TS27: medulla oblongata	Present
TS27: pons	Present
TS27: neocortex	Weak

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Strong
TS19: nasal process	Strong
TS19: genital tubercle	Present
TS19: limb	Present
TS19: lung	Present
TS19: tongue	Present
TS19: intestine	Weak
TS19: stomach	Weak
TS19: cardiac muscle tissue	Absent
TS19: liver	Absent

Structure	Level	Pattern	Image	Note
TS21: eye	Strong
TS21: metanephros	Strong
TS21: retina	Strong
TS21: genital tubercle	Present
TS21: limb	Present
TS21: tongue	Present
TS21: esophagus	Weak
TS21: inner ear	Weak
TS21: intestine	Weak
TS21: lung	Weak
TS21: olfactory epithelium	Weak
TS21: skeletal muscle	Weak
TS21: skeleton	Weak
TS21: stomach	Weak
TS21: trachea	Weak
TS21: vibrissa follicle	Weak
TS21: cardiac muscle tissue	Absent
TS21: liver	Absent
TS21: oral cavity	Absent
TS21: skin	Absent
TS21: blood vessel	Absent			Walls of large vessels.

Structure	Level	Pattern	Image	Note
TS23: eye	Strong
TS23: pituitary gland	Strong
TS23: retina	Strong
TS23: intestine	Present
TS23: metanephros	Present
TS23: tongue	Present
TS23: urethra	Present
TS23: esophagus	Weak
TS23: inner ear	Weak
TS23: limb	Weak
TS23: lung	Weak
TS23: olfactory epithelium	Weak
TS23: skeletal muscle	Weak
TS23: skeleton	Weak
TS23: stomach	Weak
TS23: tooth	Weak
TS23: trachea	Weak
TS23: vibrissa	Weak
TS23: blood vessel	Weak			Walls of large vessels.
TS23: bladder	Absent
TS23: cardiac muscle tissue	Absent
TS23: liver	Absent
TS23: oral cavity	Absent
TS23: skin	Absent

Structure	Level	Pattern	Image	Note
TS25: eye	Very strong
TS25: pituitary gland	Very strong
TS25: retina	Very strong
TS25: inner ear	Strong
TS25: olfactory epithelium	Strong
TS25: bladder	Present
TS25: esophagus	Present
TS25: metanephros	Present
TS25: skin	Present
TS25: spleen	Present
TS25: thyroid gland	Present
TS25: tooth	Present
TS25: urethra of male	Present
TS25: vibrissa follicle	Present
TS25: intestine	Weak
TS25: limb	Weak
TS25: lung	Weak
TS25: skeletal muscle	Weak
TS25: stomach	Weak
TS25: submandibular gland	Weak
TS25: thymus	Weak
TS25: tongue	Weak
TS25: trachea	Weak
TS25: blood vessel	Weak	Regionally restricted		Walls of large vessels.
TS25: brown fat	Absent
TS25: cardiac muscle tissue	Absent
TS25: liver	Absent
TS25: oral cavity	Absent
TS25: skeleton	Absent

Structure	Level	Pattern	Image	Note
TS27: adrenal gland	Strong
TS27: eye	Strong
TS27: pituitary gland	Strong
TS27: retina	Strong
TS27: bladder	Present
TS27: metanephros	Present
TS27: skin	Present
TS27: spleen	Present
TS27: inner ear	Weak
TS27: intestine	Weak
TS27: olfactory epithelium	Weak
TS27: stomach	Weak
TS27: thymus	Weak
TS27: tooth	Weak
TS27: brown fat	Absent
TS27: cardiac muscle tissue	Absent
TS27: limb	Absent
TS27: liver	Absent
TS27: lung	Absent
TS27: skeletal muscle	Absent
TS27: skeletal system	Absent
TS27: submandibular gland	Absent
TS27: tongue	Absent
TS27: vibrissa follicle	Absent
TS27: oral region	Absent			oral cavity
TS27: blood vessel	Absent			Walls of large vessels.