Probe:

ptc probe

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted		Expression first detected faintly along the anterior axis.

Structure	Level	Pattern	Image	Note
TS12: head	Strong	Regionally restricted	3A	Intense expression down the middle of headfolds.
TS12: neural ectoderm	Weak	Regionally restricted	3A	Weak expression in the neuroepithelium extending to the node.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	3C	Expression along midline of neural tube.
TS13: foregut diverticulum	Present		3C	Expression in the foregut.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	3E	Two transverse domains of expression in the developing brain.
TS13: lateral plate mesoderm	Strong	Regionally restricted	3E	High levels of expression in ventral halves of somites and lateral plate mesoderm.
TS13: somite	Strong	Regionally restricted	3E	High levels of expression in ventral halves of somites and lateral plate mesoderm.
TS13: trunk unsegmented mesenchyme	Weak	Regionally restricted	3E	Low levels of expression in ventral presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS14: floor plate	Weak		3G	Authors report that expression begins to disappear from the floor plate.
TS14: rhombomere	Present		3G
TS14: branchial arch	Present		3G
TS14: foregut	Present		3G
TS14: hindgut diverticulum	Present		3G

Structure	Level	Pattern	Image	Note
TS14: gut epithelium	Present			Expression in both endoderm and mesoderm.
TS14: gut mesenchyme	Present			Expression in both endoderm and mesoderm.

Structure	Level	Pattern	Image	Note
TS15: rhombomere	Present	Regionally restricted	3G inset	Strong stripes of expression extending dorsolaterally within each rhombomere.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	3I	Expression in the brain largely confined to the ventral midline, except for a region in the diencephalon.
TS17: diencephalon	Present	Regionally restricted	3I	Two ventrolateral stripes of expression on either side of the midline.
TS17: midbrain	Present	Regionally restricted	3I	Authors report ventrolateral expression in the midbrain.
TS17: neural tube	Present	Regionally restricted	3I	Widespread expression in the spinal cord, extending up the sides of the neural tube.
TS17: floor plate	Absent		3I	No expression in the floor plate along the entire anterior-posterior axis.
TS17: 1st branchial arch mandibular component	Present		3I
TS17: branchial arch	Present		3I
TS17: forelimb bud	Present	Regionally restricted	3I	Expression on posterior edge of forelimb bud, stronger and broader than that seen at E9.5.

Structure	Level	Pattern	Image	Note
TS12: neural tube floor plate	Strong	Regionally restricted	4A	In the presomitic region, strong expression in the ventral-most cells of the neural tube.
TS12: trunk unsegmented mesenchyme	Weak	Regionally restricted	4A	Weak expression in the ventral presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: tail neural tube	Present	Regionally restricted	4C	In the tail region, gradient of expression in the neural tube.
TS15: tail neural tube floor plate	Strong	Regionally restricted	4C	Highest levels in the floor plate adjacent to the notochord.

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Strong	Regionally restricted	4E	In the midgut region, strongest expression in cells close to the notochord.
TS15: trunk sclerotome	Weak		4E	Lower expression levels in cells more distant from the notochord.
TS15: neural tube	Present	Regionally restricted	4E	Expression persists in regions along both sides of the floor plate.
TS15: neural tube floor plate	Absent		4E

Structure	Level	Pattern	Image	Note
TS21: gut	Present		5A
TS21: skin	Present	Regionally restricted	5A	Expression in hair and whisker follicles, in rings surrounding a central domain of Shh expression.
TS21: vibrissa follicle	Present	Regionally restricted	5A	Expression in hair and whisker follicles, in rings surrounding a central domain of Shh expression.

Structure	Level	Pattern	Image	Note
TS21: gut mesenchyme	Present		5C	Authors state that expression in the gut is limited to mesoderm.
TS21: gut epithelium	Absent		5C	Authors state that expression in the gut is limited to mesoderm.

Structure	Level	Pattern	Image	Note
TS21: brain ventricular layer	Present		5E	Expression in the ventricular zone.
TS21: brain	Present	Regionally restricted	5E	Expression in two lines flanking the zona limitans.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted		Expression on the posterior edge of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	6A	Expression on posterior edge, stronger and broader than that seen at E9.5.
TS17: forelimb bud apical ectodermal ridge	Absent		6A

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Weak	Regionally restricted		Low levels of expression at the posterior edge.
TS17: hindlimb bud apical ectodermal ridge	Absent

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present	Regionally restricted	6C	Widespread expression in the posterior half.
TS18: forelimb bud apical ectodermal ridge	Absent		6C

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	6E	Expression fades in a distal domain near the edge of the limb bud.
TS19: handplate apical ectodermal ridge	Absent		6E

Structure	Level	Pattern	Image	Note
TS20: hand mesenchyme	Present	Regionally restricted	6G	Expression at the periphery of digital condensations.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted		Expression in the normal posterior domain, and in the proximal region of ectopic outgrowth.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	6I	Expression in the normal posterior domain, and in the proximal region of ectopic outgrowth, stronger in the hindlimb.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	6J	Expression in the normal posterior domain, and in the proximal region of ectopic outgrowth, stronger in the hindlimb.