Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol: Pold1tm1Uchi Name: polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1, Arikuni Uchimura MGI ID: MGI:3833573 Synonyms: Pold1- Gene: Pold1  Location: Chr7:44182168-44198239 bp, - strand  Genetic Position: Chr7, 28.83 cM Alliance: Pold1tm1Uchi page

Impaired proliferation of Pold1tm1Uchi/Pold1tm1Uchi blastocysts Show the 4 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:144992
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	C57BL/6J

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: An A to C transversion in exon 10 causes an aspartic acid to alanine amino acid residue change at codon 400. A floxed fragment containing the SV40 splice/polyadenylation signal as well as a neomycin resistance cassette was inserted in intron 10. Western blot analysis of extracts prepared from E12.5 heterozygous whole embryos confirmed that protein product was reduced by about half. (J:144992)

Generation of Pold1tm1Uchi and Pold1tm1.1Uchi alleles

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pold1 Mutation:	62 strains or lines available

Original:	J:144992 Uchimura A, et al., DNA polymerase delta is required for early mammalian embryogenesis. PLoS One. 2009;4(1):e4184
All:	1 reference(s)