Phenotypes associated with this allele

• Allele Symbol: Pold1^tm1Uchi
• Allele Name: targeted mutation 1, Arikuni Uchimura
• Allele ID: MGI:3833573
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pold1^tm1Uchi/Pold1^tm1Uchi	C57BL/6-Pold1^tm1Uchi	MGI:3833589
ht2	Pold1^tm1Uchi/Pold1^tm1.1Uchi	C57BL/6-Pold1^tm1Uchi/Pold1^tm1.1Uchi	MGI:3833591

Genotype
MGI:3833589

hm1

• Allelic Composition: Pold1^tm1Uchi/Pold1^tm1Uchi
• Genetic Background: C57BL/6-Pold1^tm1Uchi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Impaired proliferation of Pold1^tm1Uchi/Pold1^tm1Uchi blastocysts

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:144992 )

• no homozygotes found at E7.5 or beyond

• E3.5 and E4.5 homozygotes indistinguishable from controls

• no homozygotes detected among live births

embryo

absent inner cell mass proliferation ( J:144992 )

• inner cell mass from E3.5 embryos in culture fails to proliferate

inner cell mass degeneration ( J:144992 )

• inner cell mass from E3.5 embryos in culture begins to degenerate soon after appearing

• fragmented micronuclei present suggestive of apoptosis

• effects very noticeable after 3 days in culture but not after 1 day

abnormal trophoblast layer morphology ( J:144992 )

• trophoblast cells from E3.5 embryos in culture spread more slowly than those from controls

cellular

absent inner cell mass proliferation ( J:144992 )

• inner cell mass from E3.5 embryos in culture fails to proliferate

Genotype
MGI:3833591

ht2

• Allelic Composition: Pold1^tm1Uchi/Pold1^tm1.1Uchi
• Genetic Background: C57BL/6-Pold1^tm1Uchi/Pold1^tm1.1Uchi

Tumors arise in Pold1^tm1Uchi/Pold1^tm1.1Uchi mice

mortality/aging

premature death ( J:144992 )

• prone to develop a swollen thymus and die between 3 and 8 months of age

• appear otherwise to be viable and fertile

embryo

embryo phenotype ( J:144992 )

N • E12.5 embryos are morphologically like controls

immune system

enlarged thymus ( J:144992 )

• prone to develop a swollen thymus and die between 3 and 8 months of age

neoplasm

increased tumor incidence ( J:144992 )

• mice surviving beyond 12 months tend to develop nodules on their tails

hematopoietic system

enlarged thymus ( J:144992 )

• prone to develop a swollen thymus and die between 3 and 8 months of age

endocrine/exocrine glands

enlarged thymus ( J:144992 )

• prone to develop a swollen thymus and die between 3 and 8 months of age