Gene Expression Data
Assay Details
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Assay
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Reference:
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J:11028
Roelink H, et al., Expression of two members of the Wnt family during mouse development--restricted temporal and spatial patterns in the developing neural tube. Genes Dev. 1991 Mar;5(3):381-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3831577
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Gene symbol:
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Wnt3
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Gene name:
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wingless-type MMTV integration site family, member 3
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Probe:
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Wnt3 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2A:
embryonic day 10.5
(more  )
Note: Sagittal section.
Specimen
2A:
(close  )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Sagittal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: central nervous system |
Present |
Regionally restricted |
2A
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Expression in the dorsal portion of all regions of the neural tube, continuous along its length, except in the hindbrain and telencephalon.
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| TS17: neural tube |
Present |
Regionally restricted |
2A
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Expression in the dorsal portion of all regions of the neural tube.
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| TS17: hindbrain |
Present |
Regionally restricted |
2A
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Expression in the junction between the neuroepithelium and roof of myelencephalon.
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| TS17: diencephalon |
Present |
Regionally restricted |
2A
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Broad expression in dorsal portion of midbrain and diencephalon.
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| TS17: midbrain |
Present |
Regionally restricted |
2A
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Broad expression in dorsal portion of midbrain and diencephalon.
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| TS17: forebrain |
Present |
Regionally restricted |
2A
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Rostral boundary of expression lies in the telencephalic flexure, which marks the division between the diencephalon and telencephalon.
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| TS17: 1st branchial arch mandibular component |
Present |
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2A
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Authors report expression in the branchial arches.
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Specimen
2C:
embryonic day 10.5
(more )
Note: Transverse section.
Specimen
2C:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Transverse section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2C
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Expression in the junction between the neuroepithelium and roof of myelencephalon.
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| TS17: hindlimb bud |
Present |
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2C
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3A:
embryonic day 11.5
(more )
Note: Mid sagittal section.
Specimen
3A:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Mid sagittal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: central nervous system |
Present |
Regionally restricted |
3A
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Broad expression in the dorsal portion of the neural tube.
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Specimen
3D:
embryonic day 11.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3D
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Rostral boundary of expression in the dorsal thalamus.
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Specimen
3G:
embryonic day 11.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3G
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Very sharp anterior edge of expression, just caudal of the telencephalic flexure; indistinct lateral edges.
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Specimen
3J:
embryonic day 11.5
(more )
Specimen
3J:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3J
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Very sharp anterior edge of expression, just caudal of the telencephalic flexure; indistinct lateral edges.
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Specimen
3M:
embryonic day 11.5
(more )
Note: Lateral sagittal section.
Specimen
3M:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Lateral sagittal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myelencephalon |
Present |
Regionally restricted |
3M
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Expression surrounds the roof of the myelencephalon
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Specimen
4A:
embryonic day 12.5
(more )
Note: Sagittal section.
Specimen
4A:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Sagittal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
4A
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Rostral boundary of expression just behind the telencephalic flexure.
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| TS20: thalamus |
Strong |
Regionally restricted |
4A
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Strong expression in the dorsal thalamus.
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| TS20: midbrain |
Weak |
Regionally restricted |
4A
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Low level of expression in the dorsal portion of the mesencephalon.
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| TS20: cerebellum primordium |
Weak |
Regionally restricted |
4A
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Low level of expression in the cerebellum.
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Specimen
4C:
embryonic day 12.5
(more )
Note: Transverse section.
Specimen
4C:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Paraffin
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|
Note:
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Transverse section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Strong |
Regionally restricted |
4C
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Strong expression in the dorsal thalamus.
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Specimen
4E:
embryonic day 12.5
(more )
Note: Transverse section.
Specimen
4E:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Paraffin
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|
Note:
|
Transverse section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Strong |
Regionally restricted |
4E
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Strong expression in the dorsal thalamus.
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| TS20: medulla oblongata roof |
Weak |
Regionally restricted |
4E
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Decreased expression around the myelencephalic roof.
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Specimen
5A:
embryonic day 12.5
(more )
Note: Transverse section.
Specimen
5A:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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|
Embedding:
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Paraffin
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Note:
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Transverse section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: alar columns |
Present |
Regionally restricted |
5A
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Broad area of expression in the alar laminae.
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| TS20: spinal cord |
Present |
Regionally restricted |
5A
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Expression ventral horns.
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Specimen
5C:
embryonic day 12.5
(more )
Note: Parasagittal section through anterior part of spinal cord.)
Specimen
5C:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Parasagittal section through anterior part of spinal cord.)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
5C
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Expression ventral horns.
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Specimen
5E:
embryonic day 12.5
(more )
Note: Parasagittal section through ventral part of embryo.)
Specimen
5E:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Parasagittal section through ventral part of embryo.)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: skin |
Present |
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5E
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Authors report general expression throughout the ectoderm. Relatively low levels of expression in ectoderm covering the backs.
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| TS20: limb epithelium |
Strong |
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5E
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Higher levels of expression in ectoderm covering the limb buds
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| TS20: mandibular process |
Strong |
Regionally restricted |
5E
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Higher levels of expression in the caudal position of ectoderm covering the mandibular arch.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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either: BALB/c or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: oral epithelium |
Absent |
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No expression in the epithelium lining the oral cavity.
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