Antibody:	WOX1(N1)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The antibody, WOX1(N2) (MGI:3707185) gave similar results.

Structure	Level	Pattern	Image	Note
TS13: future brain	Trace		1A
TS13: heart	Strong		1A

Structure	Level	Pattern	Image	Note
TS15: future brain	Present		1B
TS15: heart	Strong		1B

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	1C	Expression was detected in ventral regions of the brainstem.
TS20: midbrain	Present	Regionally restricted	1C	Expression was detected in ventral regions of the brainstem.
TS20: spinal cord	Present	Regionally restricted	1C	Expression was detected in the ventral region.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1D	Expression was detected in the ventral portion of the basal ganglia.
TS20: cortical plate	Strong	Regionally restricted	1D	Expression was high in a densely packed zone with postmitotic cells in the cortical plate.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	1E	Expression was detected in numerous neurons and fibers in the ventral regions of the brainstem.
TS20: midbrain	Present	Regionally restricted	1E	Expression was detected in numerous neurons and fibers in the ventral regions of the brainstem.
TS20: pituitary gland	Weak		1E

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		1F
TS20: metencephalon roof	Present		1F

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata marginal layer	Present		1G
TS20: medulla oblongata ventricular layer	Present		1G
TS20: spinal cord	Present		1G

Structure	Level	Pattern	Image	Note
TS25: brain	Present		2A	Expression in the brain and spinal cord was reduced compared to E12.5 specimens.
TS25: spinal cord	Present		2A	Expression in the brain and spinal cord was reduced compared to E12.5 specimens.

Structure	Level	Pattern	Image	Note
TS25: anterior horn of lateral ventricle choroid plexus	Strong		2B
TS25: posterior horn of lateral ventricle choroid plexus	Strong		2B

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present		2C
TS25: hindbrain	Present	Regionally restricted	2C	Expression was detected in the ventral region of the brainstem.
TS25: midbrain	Present	Regionally restricted	2C	Expression was detected in the ventral region of the brainstem.
TS25: midbrain ventricular layer	Present		2C	Expression was detected in ependymal cells adjoining the ventricles.

Structure	Level	Pattern	Image	Note
TS25: vertebra	Strong	Regionally restricted	2D	Expression was high in vertebral bodies during bone formation derived from cartilage.
TS25: spinal cord	Present	Regionally restricted	2D	Expression was detected in the ventral horn.

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present	Regionally restricted	3A	Expression was detected in nerve bundles in the metencephalon.

Structure	Level	Pattern	Image	Note
TS25: trigeminal ganglion	Present		3B

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present	Regionally restricted	3C	Expression was detected in the ventral spinal cord and intermediate zones.
TS25: dorsal root ganglion	Present		3C	Expression was detected in the dorsal root ganglion and the dorsal root.

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Present		3E

Structure	Level	Pattern	Image	Note
TS26: spinal ganglion	Present		3F	Expression was localized in the cytoplasm of spinal ganglion cells.

Structure	Level	Pattern	Image	Note
TS26: spinal ganglion	Present	Regionally restricted	3G	Expression was detected in a sympathetic ganglion.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme derived from neural crest	Strong		4A
TS19: retina	Weak		4A

Structure	Level	Pattern	Image	Note
TS25: skeletal muscle	Strong	Regionally restricted	4B	Expression was intense in muscular fibers.
TS25: skeleton	Strong		4B

Structure	Level	Pattern	Image	Note
TS25: retina	Present	Regionally restricted	4C	Expression was detected in future retinal ganglion cells.

Structure	Level	Pattern	Image	Note
TS25: inner ear epithelium	Present	Regionally restricted	4D	Expression was detected in the sensory hair cells on the epithelium of the otic vesicle.
TS25: temporal bone petrous part	Strong	Regionally restricted	4D	Expression was high in the region of ossification surrounding the developing ear.

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present		6A

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present		6B	Expression was detected as in wild type with no differences noted in pattern or intensity.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6C

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		6D	Expression was detected as in wild type with no differences noted in pattern or intensity.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak			Expression was not detected in all experimental animals.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Weak			Expression was not detected in all experimental animals.
TS17: future spinal cord	Weak	Regionally restricted		Expression in the ventral horn of the spinal cord was not detected in all experimental animals.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Weak
TS20: dorsal root ganglion	Weak
TS20: corpus striatum	Weak			Expression was not detected in all experimental animals.
TS20: medulla oblongata	Weak			Expression was not detected in all experimental animals.
TS20: remnant of Rathke's pouch	Absent
TS20: midbrain	Weak	Regionally restricted		Expression was detected ventrally.
TS20: pons	Weak	Regionally restricted		Expression was detected ventrally.
TS20: spinal cord	Weak	Non-Uniform		Expression was detected in the ventral horn, but expression in the dorsal horn was not detected in all experimental animals.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Weak
TS22: choroid plexus	Weak
TS22: corpus striatum	Weak
TS22: cerebellum	Weak			Expression was not detected in all experimental animals.
TS22: olfactory lobe	Weak			Expression was not detected in all experimental animals.
TS22: thalamus	Weak			Expression was not detected in all experimental animals.
TS22: remnant of Rathke's pouch	Absent
TS22: midbrain	Weak	Regionally restricted		Expression was detected in ventral midbrain but not in the tectum.
TS22: pons	Present	Regionally restricted		Expression was detected in ventral pons.
TS22: dorsal root ganglion	Present
TS22: medulla oblongata	Present
TS22: spinal cord	Present	Non-Uniform		Expression was detected in ventral horn but was weak in the dorsal horn.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Weak
TS24: corpus striatum	Weak
TS24: medulla oblongata	Weak
TS24: olfactory lobe	Weak
TS24: thalamus	Weak
TS24: telencephalon	Weak	Regionally restricted		Expression in the limbic system was not detected in all experimental animals.
TS24: cerebellum	Weak			Expression was not detected in all experimental animals.
TS24: hypothalamus	Weak			Expression was not detected in all experimental animals.
TS24: midbrain	Present	Non-Uniform		Expression was detected in the ventral midbrain but was weak in the tectum and cerebral peduncles.
TS24: pons	Present	Regionally restricted		Expression was detected in the ventral part.
TS24: choroid plexus	Present
TS24: dorsal root ganglion	Present
TS24: spinal cord	Present	Non-Uniform		Expression was detected in ventral horn but was weak in the dorsal horn.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Weak
TS26: cerebral cortex	Weak
TS26: corpus striatum	Weak
TS26: hypothalamus	Weak
TS26: thalamus	Weak
TS26: epithalamus	Weak			Expression was not detected in all experimental animals.
TS26: medulla oblongata	Weak			Expression was not detected in all experimental animals.
TS26: olfactory lobe	Weak			Expression was not detected in all experimental animals.
TS26: telencephalon	Weak	Regionally restricted		Expression in the limbic system was not detected in all experimental animals.
TS26: diencephalon	Weak	Regionally restricted		Expression in the subthalamus was not detected in all experimental animals.
TS26: midbrain	Present	Regionally restricted		Expression was detected in the ventral midbrain and was weak in the tectum and cerebral peduncles.
TS26: pons	Weak	Regionally restricted		Expression was detected in the ventral part.
TS26: choroid plexus	Strong
TS26: spinal cord	Present	Non-Uniform		Expression was detected in the ventral horn but was weak in the dorsal horn.
TS26: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Weak
TS27: cerebral cortex	Weak
TS27: corpus striatum	Weak
TS27: hypothalamus	Weak
TS27: medulla oblongata	Weak
TS27: spinal cord ventral horn	Weak
TS27: telencephalon	Weak	Regionally restricted		Expression was weak in the limbic system.
TS27: epithalamus	Weak			Expression was not detected in all experimental animals.
TS27: spinal cord dorsal horn	Weak			Expression was not detected in all experimental animals.
TS27: subthalamic nucleus	Weak			Expression was not detected in all experimental animals.
TS27: tectum	Weak			Expression was not detected in all experimental animals.
TS27: thalamus	Weak			Expression was not detected in all experimental animals.
TS27: midbrain	Weak	Regionally restricted		Expression was detected in ventral midbrain and in the cerebral peduncles.
TS27: pons	Weak	Regionally restricted		Expression was detected in the ventral part.
TS27: choroid plexus	Strong
TS27: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present
TS28: corpus striatum	Present
TS28: dorsal root ganglion	Present
TS28: telencephalon	Weak	Regionally restricted		Expression was weak in the limbic system.
TS28: medulla oblongata	Weak			Expression was not detected in all experimental animals.
TS28: spinal cord dorsal horn	Weak			Expression was not detected in all experimental animals.
TS28: spinal cord ventral horn	Weak			Expression was not detected in all experimental animals.
TS28: subthalamic nucleus	Weak			Expression was not detected in all experimental animals.
TS28: tectum	Weak			Expression was not detected in all experimental animals.
TS28: thalamus	Weak			Expression was not detected in all experimental animals.
TS28: cerebellum	Weak
TS28: epithalamus	Weak
TS28: hypothalamus	Weak
TS28: choroid plexus	Strong
TS28: midbrain	Present	Regionally restricted		Expression was detected in the cerebral peduncles. Expression in the ventral midbrain was not detected in all experimental animals.
TS28: pons	Weak	Regionally restricted		Expression in the ventral part of the pons was not detected in all experimental animals.