Probe:	MTF#208
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Authors did not specify which probe was used when staining the specimen in Fig. S5; it is possible MTF#217 (MGI:3507577) was used.

Structure	Level	Pattern	Image	Note
TS21: head	Present		MTF# E 208 Image	Expression was detected in neural and non-neural tissue.
TS21: central nervous system	Present	Single cells	MTF# E 208 Image	Expression was detected in some cells.
TS21: cerebral cortex	Present	Single cells	MTF# E 208 Image	Expression was detected in some cells.
TS21: corpus striatum	Present	Single cells	MTF# E 208 Image	Expression was detected in some cells.
TS21: thalamus	Present	Single cells	MTF# E 208 Image	Expression was detected in some cells.
TS21: hypothalamus	Present	Single cells	MTF# E 208 Image	Expression was detected in some cells.
TS21: midbrain	Present	Single cells	MTF# E 208 Image	Expression was detected in some cells.
TS21: hindbrain	Present	Single cells	MTF# E 208 Image	Expression was detected in some cells.
TS21: spinal cord	Absent		MTF# E 208 Image
TS21: cranial ganglion	Absent		MTF# E 208 Image
TS21: dorsal root ganglion	Absent		MTF# E 208 Image
TS21: brain ventricular layer	Absent		MTF# E 208 Image
TS21: retina	Absent		MTF# E 208 Image
TS21: ear	Absent		MTF# E 208 Image	The tissue was inner ear and ear structures.
TS21: olfactory lobe	Absent		MTF# E 208 Image	The tissue was olfactory epithelium and/or bulb.

Structure	Level	Pattern	Image	Note
TS27: head	Present	Regionally restricted	MTF# P 208 Image	Expression was neural specific.
TS27: central nervous system	Present	Single cells	MTF# P 208 Image	Expression was detected in some cells.
TS27: cerebral cortex	Present	Single cells	MTF# P 208 Image	Expression was detected in some cells.
TS27: corpus striatum	Absent		MTF# P 208 Image
TS27: thalamus	Absent		MTF# P 208 Image
TS27: hypothalamus	Absent		MTF# P 208 Image
TS27: midbrain	Absent		MTF# P 208 Image
TS27: cerebellum	Absent		MTF# P 208 Image
TS27: hindbrain	Present	Single cells	MTF# P 208 Image	Expression was detected in some cells.
TS27: spinal cord	Present	Single cells	MTF# P 208 Image	Expression was detected in some cells.
TS27: cranial ganglion	Absent		MTF# P 208 Image
TS27: dorsal root ganglion	Absent		MTF# P 208 Image
TS27: brain ventricular layer	Absent		MTF# P 208 Image
TS27: retina	Present		MTF# P 208 Image , S5_Pou3f1	Based on author communication, information in Fig. S5 supersedes the supplemental table S4.
TS27: ear	Absent		MTF# P 208 Image	The tissue was inner ear and ear structures.
TS27: main olfactory bulb	Absent		MTF# P 208 Image	The tissue was olfactory epithelium and/or bulb.