Gene Expression Data
Assay Details
Assay
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Reference:
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J:73288
Sonnenberg-Riethmacher E, et al., Development and degeneration of dorsal root ganglia in the absence of the HMG-domain transcription factor Sox10. Mech Dev. 2001 Dec;109(2):253-65
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3039463
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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Anti-NF-160
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
5A:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
Specimen
5A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Sox10tm1Weg/Sox10+
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dorsal root ganglion |
Present |
Regionally restricted |
5A
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Cells that express Nef3 do not express lacZ and vice versa.
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Specimen
5B:
embryonic day 11.5; Sox10tm1Weg/ Sox10tm1Weg
(more )
Note: Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
Specimen
5B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Sox10tm1Weg/Sox10tm1Weg
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Age:
|
embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dorsal root ganglion |
Present |
Regionally restricted |
5B
|
Cells that express Nef3 do not express lacZ and vice versa.
|
Specimen
5G:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: green - Nefm; red - Fabp7 (assay MGI:3039705). Embryos were fixed in 1% paraformaldehyde.
Specimen
5G:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Sox10tm1Weg/Sox10+
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: green - Nefm; red - Fabp7 (assay MGI:3039705). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
5H:
embryonic day 11.5; Sox10tm1Weg/ Sox10tm1Weg
(more )
Note: Double labeled: green - Nefm; red - Fabp7 (assay MGI:3039705). Embryos were fixed in 1% paraformaldehyde.
Specimen
5H:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10tm1Weg
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Nefm; red - Fabp7 (assay MGI:3039705). Embryos were fixed in 1% paraformaldehyde.
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Specimen
6C:
embryonic day 11.5; Sox10tm1Weg/Sox10 +
(more )
Note: Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
Specimen
6C:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Sox10tm1Weg/Sox10+
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
|
Specimen
6D:
embryonic day 11.5; Sox10tm1Weg/ Sox10tm1Weg
(more )
Note: Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
Specimen
6D:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
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Sox10tm1Weg/Sox10tm1Weg
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Nefm; red - Sox10 reporter (assay MGI:3039657). Embryos were fixed in 1% paraformaldehyde.
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