Gene Expression Data
Assay Details
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Assay
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Reference:
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J:83431
Morgan EA, et al., Loss of Bmp7 and Fgf8 signaling in Hoxa13-mutant mice causes hypospadia. Development. 2003 Jul;130(14):3095-109
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:2681891
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Gene symbol:
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Hoxa13
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Gene name:
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homeobox A13
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Reporter:
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GFP
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Direct Detection
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Results
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Specimen
1A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: genital tubercle |
Present |
Regionally restricted |
1A
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Expression was detected in the genital tubercle mesenchyme and the urethral plate epithelium.
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: genital tubercle |
Present |
Regionally restricted |
1B
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Expression was detected in the genital tubercle mesenchyme and the urethral plate epithelium and was not different from the heterozygous embryo.
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Specimen
1E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: male reproductive system |
Present |
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1F
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No difference in expression was noted between heterozygous and homozygous embryos.
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| TS23: urethra of male |
Present |
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1F
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No difference in expression was noted between heterozygous and homozygous embryos.
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Specimen
2A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: genital tubercle |
Present |
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2B
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No difference in expression was noted between heterozygous and homozygous embryos.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
|
|
Age:
|
embryonic day 12.5
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|
Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: genital tubercle |
Present |
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2D
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No difference in expression was noted between heterozygous and homozygous embryos.
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Specimen
6J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 11.5
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Sex:
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Male
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|
Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: genital tubercle |
Present |
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6L
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No difference in expression was noted between heterozygous and homozygous embryos.
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Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
|
|
Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: reproductive system |
Present |
Regionally restricted |
7C
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Expression was detected in the urethral plate epithelium.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 12.5
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Sex:
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Male
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|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: reproductive system |
Present |
Regionally restricted |
7D
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Expression was detected in the urethral plate epithelium and was not different from the heterozygous embryo.
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Specimen
7E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Male
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|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: reproductive system |
Present |
Regionally restricted |
7E
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Expression was detected in the urethral plate epithelium.
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Specimen
7F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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|
Age:
|
embryonic day 12.5
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|
Sex:
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Male
|
|
Type:
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section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: reproductive system |
Present |
Regionally restricted |
7F
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Expression was detected in the urethral plate epithelium and was not different from the heterozygous embryo.
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Specimen
8A:
embryonic day 13.5; Hoxa13tm1.1Mrc/Hoxa13 +
(more  )
Note: Double labeled: green - Hoxa13 reporter; red - Pecam1 (assay MGI:2675829).
Specimen
8A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxa13 reporter; red - Pecam1 (assay MGI:2675829).
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Specimen
8B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxa13 reporter; red - Pecam1 (assay MGI:2675829).
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Specimen
9A:
embryonic day 15.5; Hoxa13tm1.1Mrc/Hoxa13 +
(more )
Note: Double labeled: green - Hoxa13 reporter; red - Ar (assay MGI:2675828).
Specimen
9A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
|
|
Age:
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embryonic day 15.5
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|
Sex:
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Male
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|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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|
Note:
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Double labeled: green - Hoxa13 reporter; red - Ar (assay MGI:2675828).
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Specimen
9B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
|
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Hoxa13 reporter; red - Ar (assay MGI:2675828).
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Specimen
9C:
embryonic day 15.5; Hoxa13tm1.1Mrc/Hoxa13 +
(more )
Note: Double labeled: green - Hoxa13 reporter; red - Ar (assay MGI:2675828).
Specimen
9C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13+
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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|
Note:
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Double labeled: green - Hoxa13 reporter; red - Ar (assay MGI:2675828).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: male reproductive system |
Present |
Regionally restricted |
9C
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Expression was detected in the mesenchyme immediately adjacent to the urethral plate epithelium.
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Specimen
9D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Hoxa13tm1.1Mrc/Hoxa13tm1.1Mrc
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Double labeled: green - Hoxa13 reporter; red - Ar (assay MGI:2675828).
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