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Gene Expression Data
Assay Details
Assay
Reference: J:48293 Reule M, et al., Analysis of Peg1/Mest imprinting in the mouse. Dev Genes Evol. 1998 May;208(3):161-3
Assay type: RT-PCR
MGI Accession ID: MGI:2681478
Gene symbol: Mest
Gene name: mesoderm specific transcript
Probe: Mest-pA, Mest-pB
Assay notes: The results of this assay were not shown. The RT-PCR sample was digested with the restriction endonuclease TaqI prior to electrophoresis. The mus allele gives three bands of 284, 173 and 30bp. The spretus allele gives two bands of 284 and 203bp. Author states that there was a discrepancy in the results obtained in 2 experiments at E3.5.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
Not shown E3.5 (a) TS4: blastocyst-stage conceptus Present Weak Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Author states that this experiment indicated biallelic expression of the gene.
Not shown E3.5 (a) TS4: blastocyst-stage conceptus Present Absent Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Author states that this experiment indicated monoallelic expression of the gene.
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) This band is present in the spretus allele.
(c) This band is present in the mus allele.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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