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Gene Expression Data
Assay Details
Assay
Reference: J:48293 Reule M, et al., Analysis of Peg1/Mest imprinting in the mouse. Dev Genes Evol. 1998 May;208(3):161-3
Assay type: RT-PCR
MGI Accession ID: MGI:2681477
Gene symbol: Mest
Gene name: mesoderm specific transcript
Probe: Mest-pA, Mest-pB
Assay notes: The RT-PCR sample was digested with the restriction endonuclease TaqI prior to electrophoresis. The mus allele gives three bands of 284, 173 and 30bp. The spretus allele gives two bands of 284 and 203bp.
Results Image: 1B
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
marker 1 Mol. Wt. Marker Lane
spleen F1 P adult TS28: spleen Present Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Female mother was biallelic.
placenta E11.5 (c) TS28: placenta Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Female this tissue was monoallelic for the paternal allele.
fetus E11.5 (c) TS19: embryo Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified this tissue was monoallelic for the paternal allele.
Notes:
(a) This band was present in the spretus allele.
(b) This band was present in the mus allele.
(c) Age normalized so that noon of plug day = E0.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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