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Gene Expression Data
Assay Details
Assay
Reference: J:48293 Reule M, et al., Analysis of Peg1/Mest imprinting in the mouse. Dev Genes Evol. 1998 May;208(3):161-3
Assay type: RT-PCR
MGI Accession ID: MGI:2681476
Gene symbol: Mest
Gene name: mesoderm specific transcript
Probe: Mest-pA, Mest-pB
Assay notes: The RT-PCR sample was digested with the restriction endonuclease TaqI prior to electrophoresis. The mus allele gives three bands of 284, 173 and 30bp. The spretus allele gives two bands of 284 and 203bp.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
marker 1 Mol. Wt. Marker Lane
e10.1 E9.5 (c) TS15: embryo Present Absent Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified This embryo was heterozygous for the mus and spretus alleles.
e10.4 E9.5 (c) TS15: embryo Present Absent Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified This embryo was homozygous for the mus allele.
spleen F1 P adult TS28: spleen Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Female In the maternal tissue, only the spretus band pattern was observed.
liver F1 P adult TS28: liver Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Female In the maternal tissue, only the spretus band pattern was observed.
Notes:
(a) This band was present in the mus allele.
(b) This band was present in the spretus allele.
(c) Age normalized so that noon of plug day = E0.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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