Probe:	Nkx-2.2 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	3A	Expression begins in front of the head process and extends near to the anterior edge of the neural plate.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	3B	Bilateral stripes of expression cross the midline and posteriorly end at the presumptive zona limitans.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	3D	Separate domains of expression are present in the forebrain, midbrain/posterior diencephalon, hindbrain, and anterior spinal cord.
TS13: future midbrain	Present	Regionally restricted	3D	Separate domains of expression are present in the forebrain, midbrain/posterior diencephalon, hindbrain, and anterior spinal cord.
TS13: future prosencephalon	Present	Regionally restricted	3D	Separate domains of expression are present in the forebrain, midbrain/posterior diencephalon, hindbrain, and anterior spinal cord.
TS13: future spinal cord	Present	Regionally restricted	3D	Separate domains of expression are present in the forebrain, midbrain/posterior diencephalon, hindbrain, and anterior spinal cord.

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present		3G	Expression in a stripe along the entire anterior-posterior extent of the central nervous system.
TS16: future brain	Present	Regionally restricted	3G	Expression has a dorsal deflection at the zona limitans. Expression crosses the midline just ventral to the optic stalk. Anterior to the isthmus the expression extends to the front of the brain in a more dorsal position.
TS16: future hindbrain	Present	Regionally restricted	3G	Expression has dorsal deflections at the interrhombomeric boundaries.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	3H	Expression has a dorsal deflection at the zona limitans. Expression crosses the midline just ventral to the optic stalk.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	3I	Expression at the level of the postoptic forebrain.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	3J	Expression in the caudal diencephalon.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		3K	Expression in the paramedian plate cells. Posterior to the isthmus expression is in paramedian stripes that separate the floor plate from the longitudinal columns of motor neurons.
TS19: hindbrain	Present		3K	Expression in the paramedian plate cells. Posterior to the isthmus expression is in paramedian stripes that separate the floor plate from the longitudinal columns of motor neurons.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		4A	The expression stripe is dorsal to the oculomotor nucleus and midbrain tegmentum.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		4B

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		4C

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		4D

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		4E

Structure	Level	Pattern	Image	Note
TS15: future brain	Present		5C