Gene Expression Data
Assay Details
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Assay
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Reference:
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J:31212
Shimamura K, et al., Longitudinal organization of the anterior neural plate and neural tube. Development. 1995 Dec;121(12):3923-33
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1934638
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe20
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3 C:
embryonic day 8.5
(more  )
Specimen
3 C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future spinal cord neural plate |
Present |
Regionally restricted |
3C
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Transcripts occupy a median position of the anterior neural plate, where it forms a narrow loop in the floor of the neural groove.
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Specimen
3 E:
embryonic day 8.5
(more )
Note: 12 somites.
Specimen
3 E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
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3E
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Expression extends from the anterior forebrain to a posterior limit in the anterior hindbrain.
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| TS13: future midbrain |
Present |
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3E
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Expression extends from the anterior forebrain to a posterior limit in the anterior hindbrain.
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| TS13: future prosencephalon |
Present |
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3E
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Expression extends from the anterior forebrain to a posterior limit in the anterior hindbrain.
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Specimen
3 G:
embryonic day 10.0
(more )
Note: Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
Specimen
3 G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: central nervous system |
Present |
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3G
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Expression in a stripe along the entire anterior-posterior extent of the central nervous system.
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| TS16: future brain |
Present |
Regionally restricted |
3G
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Expression has a dorsal deflection at the zona limitans. Expression crosses the midline just ventral to the optic stalk.
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| TS16: future forebrain |
Present |
Regionally restricted |
3G
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Expression in a separate domain in the medial ganglionic eminence.
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Specimen
3 H:
embryonic day 11.5
(more )
Note: Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
Specimen
3 H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: brain |
Present |
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3H
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Expression has a dorsal deflection at the zona limitans. Expression crosses the midline just ventral to the optic stalk.
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| TS19: forebrain |
Present |
Regionally restricted |
3H
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Expression in a separate domain in the medial ganglionic eminence.
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Specimen
3 I:
embryonic day 11.5
(more )
Note: Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
Specimen
3 I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forebrain |
Present |
Regionally restricted |
3I
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Expression domain at the level of the postoptic forebrain.
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Specimen
3 J:
embryonic day 11.5
(more )
Note: Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
Specimen
3 J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
3J
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Expression in the caudal diencephalon.
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Specimen
3 K:
embryonic day 11.5
(more )
Note: Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
Specimen
3 K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Shh; purple - Nkx2-2 (assay MGI:1934636).
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Specimen
5 D:
embryonic day 9.5-9.75
(more )
Specimen
5 D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5-9.75
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools