Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45301
Cygan JA, et al., Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants. Development. 1997 Dec;124(24):5021-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933251
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Gene symbol:
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Lmx1b
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Gene name:
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LIM homeobox transcription factor 1 beta
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Probe:
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Lmx1b cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 8.5
(more  )
Note: 10 somites.
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
1A
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Expression in the lateral plate mesoderm of the presumptive forelimb level.
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Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
1C
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Expression in cells approximately 9-12 cell layers beneath the dorsal ectoderm.
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Specimen
1D:
embryonic day 13.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1E:
embryonic day 14.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forearm |
Present |
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1E
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Expression levels have dropped.
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| TS22: hand mesenchyme |
Strong |
Regionally restricted |
1E
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Expression in the dorsal mesenchyme of the autopod.
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Specimen
1F:
embryonic day 14.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2A:
embryonic day 11.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
2A
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Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2A
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Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
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Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
2B
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Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2B
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Expression in distal anterior mesenchyme and around the lateral margin of the limb bud.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2C
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Up to 11.5 dpc expression is the same as in wild-type. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud. The anterior mesenchyme is more severely affected.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2D
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Up to 11.5 dpc expression is the same as in wild-type. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud.
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Specimen
2E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
2F
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Expression is similar to wild-type in the dorsal mesenchyme and an additional ectopic domain in the ventral mesenchyme.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2F
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Expression is similar to wild-type in the dorsal mesenchyme and an additional ectopic domain in the ventral mesenchyme.
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
2G
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Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud. The anterior mesenchyme is more severely affected.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2G
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Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. Expression is absent in the dorsal mesenchyme around the lateral margin of the limb bud. The anterior mesenchyme is more severely affected.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
2H
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Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. No expression in the ventral mesenchyme.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2H
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Similar expression as in Wnt7a -/- embryos, overall expression patterns are lower. No expression in the ventral mesenchyme.
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Specimen
3A:
embryonic day 14.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hand mesenchyme |
Present |
Regionally restricted |
3A
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Expression in the dorsal mesenchyme of the autopod.
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Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hand mesenchyme |
Present |
Regionally restricted |
3C
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Expression in the dorsal mesenchyme of the autopod.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3I:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3K:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5K:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Spotted |
5K
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Patchy expression in the ventral limb bud.
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Specimen
5L:
embryonic day 11.5
(more )
Note: Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
Specimen
5L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
5L
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Expression in the dorsal mesenchyme terminates at the level of Fgf8 expression in the ectoderm.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
5L
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Expression in the dorsal mesenchyme terminates at the level of Fgf8 expression in the ectoderm.
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Specimen
5M:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: orange - Lmx1b; purple - Fgf8 (assay MGI:1933301).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm mesenchyme |
Present |
Regionally restricted |
5M
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A similar correlation between the distal extent of ectopic Lmx1b expression in the ventral mesenchyme and the position of ectopic Fgf8 expression in the ventral ectoderm.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
5M
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A similar correlation between the distal extent of ectopic Lmx1b expression in the ventral mesenchyme and the position of ectopic Fgf8 expression in the ventral ectoderm.
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