Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45301
Cygan JA, et al., Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants. Development. 1997 Dec;124(24):5021-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933301
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5A:
embryonic day 11.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm ectoderm |
Present |
Regionally restricted |
5C
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Expression in the ventral ectoderm that has the morphology of an AER. This ectopic ridge of cells originates at the anterior end of the endogenous AER and often termintaes at approximately the midline of the A-P axis.
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| TS19: handplate apical ectodermal ridge |
Present |
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5C
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5E:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future arm ectoderm |
Present |
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5F
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Expression in the ventral ectoderm that has the morphology of an AER. This ectopic ridge of cells originates at the anterior end of the endogenous AER and often termintaes at approximately the midline of the A-P axis.
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| TS19: handplate apical ectodermal ridge |
Present |
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5F
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Specimen
5K:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Fgf8; orange - Lmx1b (assay MGI:1933251).
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Specimen
5L:
embryonic day 11.5
(more )
Note: Double labeled: purple - Fgf8; orange - Lmx1b (assay MGI:1933251).
Specimen
5L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Fgf8; orange - Lmx1b (assay MGI:1933251).
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Specimen
5M:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Fgf8; orange - Lmx1b (assay MGI:1933251).
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Specimen
6D:
embryonic day 11.5
(more )
Note: Double labeled: orange - Fgf8; purple - Shh (assay MGI:1933357).
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: orange - Fgf8; purple - Shh (assay MGI:1933357).
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: orange - Fgf8; purple - Shh (assay MGI:1933357).
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Analysis Tools