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Gene Expression Data
Assay Details
Assay
Reference: J:45301 Cygan JA, et al., Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants. Development. 1997 Dec;124(24):5021-32
Assay type: RNA in situ
MGI Accession ID: MGI:1933301
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 cDNA13
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 5A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present 5A
TS19: handplate apical ectodermal ridge Present 5A
Specimen 5B: embryonic day 11.5; Wnt7atm1Amc/Wnt7atm1Amc (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present 5B
TS19: handplate apical ectodermal ridge Present 5B
TS19: future arm ectoderm Present Regionally restricted 5B Expression in forelimb and a patch of cells in the dorsal ectoderm (1 of 12 embryos).
TS19: handplate ectoderm Present Regionally restricted 5B Expression in forelimb and a patch of cells in the dorsal ectoderm (1 of 12 embryos).
Specimen 5C: embryonic day 11.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS19: future arm ectoderm Present Regionally restricted 5C Expression in the ventral ectoderm that has the morphology of an AER. This ectopic ridge of cells originates at the anterior end of the endogenous AER and often termintaes at approximately the midline of the A-P axis.
TS19: handplate apical ectodermal ridge Present 5C
Specimen 5D: embryonic day 11.5; En1tm1Alj/En1tm1Alj, Wnt7atm1Amc/Wnt7atm1Amc (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present 5D Similar expression to wild-type.
Specimen 5E: embryonic day 11.5; Wnt7atm1Amc/Wnt7atm1Amc (more )
Structure Level Pattern Image Note
TS19: forelimb bud Present 5E
TS19: future arm ectoderm Present Regionally restricted 5E Expression in forelimb and a patch of cells in the dorsal ectoderm (1of 12 embryos).
TS19: handplate ectoderm Present Regionally restricted 5E Expression in forelimb and a patch of cells in the dorsal ectoderm (1of 12 embryos).
Specimen 5F: embryonic day 11.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS19: future arm ectoderm Present 5F Expression in the ventral ectoderm that has the morphology of an AER. This ectopic ridge of cells originates at the anterior end of the endogenous AER and often termintaes at approximately the midline of the A-P axis.
TS19: handplate apical ectodermal ridge Present 5F
Specimen 5K: embryonic day 11.5; En1tm1Alj/En1tm1Alj (more )
Note: Double labeled: purple - Fgf8; orange - Lmx1b (assay MGI:1933251).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5K
Specimen 5L: embryonic day 11.5 (more )
Note: Double labeled: purple - Fgf8; orange - Lmx1b (assay MGI:1933251).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5L
Specimen 5M: embryonic day 11.5; En1tm1Alj/En1tm1Alj (more )
Note: Double labeled: purple - Fgf8; orange - Lmx1b (assay MGI:1933251).
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 5M Expression in the endogenous and ectopic AER.
Specimen 6D: embryonic day 11.5 (more )
Note: Double labeled: orange - Fgf8; purple - Shh (assay MGI:1933357).
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 6D
Specimen 6E: embryonic day 11.5; En1tm1Alj/En1tm1Alj (more )
Note: Double labeled: orange - Fgf8; purple - Shh (assay MGI:1933357).
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 6E The domain of Shh expressing cells expands ventrally to the level of ectopic Fgf8 staining in the ventral ectoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory