Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45301
Cygan JA, et al., Novel regulatory interactions revealed by studies of murine limb pattern in Wnt-7a and En-1 mutants. Development. 1997 Dec;124(24):5021-32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1933357
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 11.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
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6B
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Fewer expressing cells compared to wild-type.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
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6C
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The number of expressing cells appears similar to wild-type but is reduced and the morphology of the limb bud is different.
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Specimen
6D:
embryonic day 11.5
(more )
Note: Double labeled: purple - Shh; orange - Fgf8 (assay MGI:1933301).
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Shh; orange - Fgf8 (assay MGI:1933301).
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Shh; orange - Fgf8 (assay MGI:1933301).
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Analysis Tools