Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65601
Garel S, et al., Control of the migratory pathway of facial branchiomotor neurones. Development. 2000 Dec;127(24):5297-307
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1929061
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Gene symbol:
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Ret
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Gene name:
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ret proto-oncogene
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Probe:
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pmcRet7
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2E:
embryonic day 11.75
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metencephalon |
Present |
Regionally restricted |
2E
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In the rhombomere 4 region, expression is in a narrow stripe of cells, but not in the larger population of bm neurons. As the neurons enter the rhombomere 5 region, they switch on gene expression.
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Specimen
2F:
embryonic day 12.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2G:
embryonic day 11.5
(more )
Note: Also stained for Hoxb1 (assay MGI:1928752); both blue.
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for Hoxb1 (assay MGI:1928752); both blue.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metencephalon |
Present |
Regionally restricted |
2G
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In the rhombomere 4 region, expression is in a narrow stripe of cells, but not in the larger population of bm neurons. As the neurons enter the rhombomere 5 region, they switch on gene expression.
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Specimen
3C:
embryonic day 12.0; Egr2tm1Pch/Egr2 +
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
3C
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Expression is in fmb neurons exiting the rhombomere 4 region.
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Specimen
3C':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
3C'
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Expression is in fmb neurons exiting the rhombomere 4 region.
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Specimen
3E:
embryonic day 14.5; Mafbkr/Mafb +
(more )
Specimen
3E:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Mafbkr/Mafb+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3E':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4C:
embryonic day 12.5; Egr2tm1Pch/Egr2 +
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4C':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5C:
embryonic day 14.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5C':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7D:
embryonic day 12.5
(more )
Specimen
7D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7D':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: facial ganglion |
Present |
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7D'
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| TS20: metencephalon |
Present |
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7D'
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Expression in this mutant is expanded. In addition to the normal migrating fbm neurons in rhombomere regions 5 and 6, expression extends into rhombomere region 4.
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Analysis Tools