Probe:	GATA-2 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: rhombomere 2	Present		1A
TS15: rhombomere 4	Present		1A

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	1C	Expression is detected in ventral mesencephalon in the presumptive common oculomotor nucleus.
TS15: rhombomere 4	Strong		1C
TS15: rhombomere 2	Absent		1C

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	1E	Expression is detected in the olfactory bulb.
TS17: midbrain roof plate	Present	Regionally restricted	1E	Expression is detected in the pretectum.
TS17: future spinal cord	Present	Regionally restricted	1E	Expression is detected in a ventrolateral column spanning the hindbrain and the spinal cord; this domain extends to the floor plate from its rostral limit to r4.
TS17: hindbrain	Present	Regionally restricted	1E	Expression is detected in a ventrolateral column spanning the hindbrain and the spinal cord; this domain extends to the floor plate from its rostral limit to r4.
TS17: central nervous system ganglion	Present	Regionally restricted	1E	Expression is detected in the common oculomotor nucleus.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1G	Expression is detected in the olfactory bulb.
TS19: midbrain roof plate	Present	Regionally restricted	1G	Expression is detected in the pretectum.
TS19: central nervous system ganglion	Present	Regionally restricted	1G	Expression is detected in the oculomotor nucleus.

Structure	Level	Pattern	Image	Note
TS15: rhombomere 4	Moderate	Regionally restricted	2A	Expression is detected ventrally in rhombomere 4.
TS15: future hindbrain	Weak	Regionally restricted	2A	Expression is detected in a ventral column of cells.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	2B	Expression is detected in a narrow column of cells lining the floor plate and a more lateral column, which abuts the former domain from rostral hindbrain to r4, where the expression domain is more ventrally restricted than that of Gata3.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	2C	Expression is detected ventrally downward from the mesencephalon; it is extinguished in r4.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present		2G
TS17: future spinal cord	Present	Regionally restricted	2G	Expression is detected in a ventrolateral column spanning the hindbrain and spinal cord. Isl1 expression domain overlaps the Gata2 expression domain, with more ventral cells expressing only Isl1 and more dorsal cells express Gata2 or both Isl1 and Gata2.

Structure	Level	Pattern	Image	Note
TS18: central nervous system ganglion	Present	Regionally restricted	3A	Expression is weaker in the ventricular than in the subventricular zone in the common oculomotor nucleus.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 1	Present	Regionally restricted	3B	Expression is detected throughout the mediolateral axis of the ventral neural tube in r1, with a marked activation in a cluster of cells which juxtapose the floor plate.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 4	Present	Regionally restricted	3C	Expression is detected in a ventral domain of r4, the most lateral of which extends to the floor plate.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 6	Present	Regionally restricted	3D	Expression is observed in two distinct domains: one close to the floor plate and a more dorsal one, expanding laterally throughout the neural tube.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted	3E	Expression in the spinal cord is activated ventrally in both the ventricular and subventricular zone, but is excluded from the mantle layer.

Structure	Level	Pattern	Image	Note
TS13: future brain	Absent
TS13: future spinal cord	Absent