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Gene Expression Data
Assay Details
Assay
Reference: J:56426 Bi W, et al., The transcription factor MEF2C-null mouse exhibits complex vascular malformations and reduced cardiac expression of angiopoietin 1 and VEGF. Dev Biol. 1999 Jul 15;211(2):255-67
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1342015
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody
Assay notes: Embryos were dissected and fixed in a manner that rendered the normally curved mouse embryo into a planar orientation. Staining was visualized using laser scanning confocal microscopy. Embryos were subjected to optical sectioning and the sections were then collapsed to generate a single image depicting the entire embryonic vasculature.
Results
Specimen 3A: embryonic day 8.0 (more )
Note: Once flat, embryos were fixed by the slow addition of 3% paraformaldehyde to the PBS. After the mouse embryos had been flattened and fixed, they were immunolabeled and processed.
Structure Level Pattern Image Note
TS12: cardiovascular system Present 3A
TS12: dorsal aorta Present 3A
TS12: heart Present 3A
Specimen 3B: embryonic day 8.0; Mef2ctm1Eno/Mef2ctm1Eno (more )
Note: Once flat, embryos were fixed by the slow addition of 3% paraformaldehyde to the PBS. After the mouse embryos had been flattened and fixed, they were immunolabeled and processed.
Structure Level Pattern Image Note
TS12: cardiovascular system Present 3B
TS12: dorsal aorta Present 3B
TS12: heart Present 3B
Specimen 4C: embryonic day 9.5 (more )
Note: Once flat, embryos were fixed by the slow addition of 3% paraformaldehyde to the PBS. After the mouse embryos had been flattened and fixed, they were immunolabeled and processed.
Structure Level Pattern Image Note
TS15: heart Present 4C
Specimen 4D: embryonic day 9.5; Mef2ctm1Eno/Mef2ctm1Eno (more )
Note: Once flat, embryos were fixed by the slow addition of 3% paraformaldehyde to the PBS. After the mouse embryos had been flattened and fixed, they were immunolabeled and processed.
Structure Level Pattern Image Note
TS15: heart Present 4D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/28/2026
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