Gene Expression Image Detail

Reference

Figure

ID

J:56426 Bi W, et al., Dev Biol. 1999 Jul 15;211(2):255-67

4

MGI:1341993

Image

Caption

The endothelial cells of the endocardium are malformed and have an irregular orientation relative to the myocardium. Hematoxylin and eosin staining of a normal (A) ventricle shows the extended shape of normal endothelial cells and their uniform orientation to the myocardium. The endothelial cells of the mutant (B) are rounded and clumped together (arrows). The endocardium is collapsed between the left ventricle and the outflow tract (arrowhead), and the endothelial cells also have an irregular orientation with respect to the myocardium (asterisk). In C and D, the phenotypes of normal (C) and mutant (D) endocardia as viewed by LSCM are compared. Both hearts were immunolabeled with antibodies to PECAM and were viewed from the ventral surface. In the normal heart (C), the countour of the endocardium is relatively smooth, and in general, there are few endocardial projections outward to the myocardium. In contrast, the contour of the endocardium of the mutant embryos is rough in appearance. Numerous small projections extending from the endocardium disrupt the contour of the endocardium, giving it a "spiky" appearance. The disruption of normal endothelial development is also evident from the relative absence of visible individual cell boundaries in the mutant (D) that are pronounced in the normal (C). A, atrium; EC, endothelial cells; V, ventricle.

Copyright

Reprinted with permission from Elsevier from doi:10.1006/dbio.1999.9307 Dev Biol 211: 255-67, Bi W; Drake CJ; Schwarz JJ, The transcription factor MEF2C-null mouse exhibits complex vascular malformations and reduced cardiac expression of angiopoietin 1 and VEGF. Copyright 1999

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
C	MGI:1342015 (Pecam1)
D	MGI:1342015 (Pecam1)