Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47783
Obata Y, et al., Disruption of primary imprinting during oocyte growth leads to the modified expression of imprinted genes during embryogenesis. Development. 1998 Apr;125(8):1553-60
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:1328260
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Gene symbol:
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Mest
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Gene name:
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mesoderm specific transcript
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Probe:
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Mest-pA, Mest-pB
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Assay notes:
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Amplification: 30 cycles at 95 C for 30 seconds, 65 C for 30 seconds, and 72 for 60 seconds.
ng/fg PE = parthenogenetic embryo (PE) containing genomes from non-growing (ng) and fully grown oocytes (fg).
fg/fg PE = parthenogenetic embryo containing genomes from two fully grown oocytes.
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Results
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| E9.5
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TS15: embryo
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Present
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1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
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| E9.5
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TS15: embryo
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Present
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1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
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| E9.5
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TS15: embryo
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Present
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1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
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| Treated sample: no data stored |
| Treated sample: no data stored |
| Treated sample: no data stored |
| Treated sample: no data stored |
| E12.5
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TS20: embryo
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Present
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1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
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| E12.5
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TS20: embryo
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Present
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1.00 µg; total RNA |
(C57BL/6J x CBA)F1 |
| Not Specified |
biparental embryo
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| Treated sample: no data stored |
| Treated sample: no data stored |
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