Probe:	Bmp7 cDNA5
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: surface ectoderm	Weak	Regionally restricted	1D	Expression limited to dorsal surface ectoderm.
TS13: head mesenchyme	Weak		1D
TS13: foregut diverticulum	Present		1D
TS13: notochord	Present		1D
TS13: neural ectoderm	Present	Regionally restricted	1D	Expression limited to most anterior neurectoderm.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1I	Expression is limited to most anterior ectoderm.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	1O	Expression is restricted to the most anterior dorsal neurectoderm.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	2D	Expression is detected in the dorsal forebrain.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2J	Expression detected in the anteriormost dorsal roof of the telencephalon.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	3C	Expression is detected in the dorsomedial part of the telencephalon and the dorsalmidline of the anterior diencephalon. Transcripts are also detected in the dorsal hindbrain.
TS17: hindbrain	Present	Regionally restricted	3C	Expression is detected in the dorsomedial part of the telencephalon and the dorsalmidline of the anterior diencephalon. Transcripts are also detected in the dorsal hindbrain.
TS17: telencephalon	Present	Regionally restricted	3C	Expression is detected in the dorsomedial part of the telencephalon and the dorsalmidline of the anterior diencephalon. Transcripts are also detected in the dorsal hindbrain.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4D	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.
TS19: metencephalon roof	Present	Regionally restricted	4D	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.
TS19: myelencephalon roof plate	Present	Regionally restricted	4D	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.
TS19: telencephalon	Present	Regionally restricted	4D	Expression is detected in the dorsomedial telencephalon, the dorsal diencephalon and the roofplate of the hindbrain.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4I	Expression is detected in the roof between the cerebral hemispheres.

Structure	Level	Pattern	Image	Note
TS21: 3rd ventricle	Present	Regionally restricted	4N	Expression is detected in the medial walls of the lateral ventricle, the prospective hippocampus.
TS21: 4th ventricle	Present	Regionally restricted	4N	Expression is detected in the medial walls of the lateral ventricle, the prospective hippocampus.
TS21: lateral ventricle	Present	Regionally restricted	4N	Expression is detected in the medial walls of the lateral ventricle, the prospective hippocampus.
TS21: lateral ventricle choroid plexus	Strong	Uniform	4N
TS21: metencephalon part of 4th ventricle choroid plexus	Strong	Uniform	4N

Structure	Level	Pattern	Image	Note
TS16: future brain	Present		5L	Expression pattern is similar to Hfh4.