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Gene Expression Data
Assay Details
Assay
Reference: J:39351 de la Pompa JL, et al., Conservation of the Notch signalling pathway in mammalian neurogenesis. Development. 1997 Mar;124(6):1139-48
Assay type: Western blot
MGI Accession ID: MGI:1267245
Gene symbol: Rbpj
Gene name: recombination signal binding protein for immunoglobulin kappa J region
Antibody: RBPJK antibody
Detection system: Protein A coupled to horseradish peroxidase
Assay notes: The lower band on the blot is most likely the result of alternative splicing of the RBP-Jkappa primary transcript or a proteolytic fragment of RBPJK. Band presence/absence not discussed; in the figure, it appears to be expressed in all stages but -/- at E8.5 and E9.5.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 60 kDa (a) Genetic BackgroundMutant Allele(s)Sex Note
7.5 E7.5 TS11: embryo Present Not Specified Not Specified Protein was present in the nuclear fraction.
+/+ 8.5 E8.5 TS13: embryo Present Not Specified Not Specified Protein was present in the nuclear fraction.
+/- 8.5 E8.5 TS13: embryo Moderate involves: 129S2/SvPas * C57BL/6 Rbpjtm1Kyo/Rbpj+Not Specified Protein was present in the nuclear fraction.
-/- 8.5 E8.5 TS13: embryo Absent involves: 129S2/SvPas * C57BL/6 Rbpjtm1Kyo/Rbpjtm1KyoNot Specified
9.5 E9.5 TS15: embryo Present Not Specified Not Specified Protein was present in the nuclear fraction.
10.5 E10.5 TS17: embryo Present Not Specified Not Specified Protein was present in the nuclear fraction.
11.5 E11.5 TS19: embryo Present Not Specified Not Specified Protein was present in the nuclear fraction.
12.5 E12.5 TS20: embryo Present Not Specified Not Specified Protein was present in the nuclear fraction.
Notes:
(a) This band represents the full-length RBPJK protein.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory