Fragment was generated by PCR with primers CGCGGATCCTGTGAGGCTCCTCTCAGCTGC and CCGCTCGAGCAGCTGGTCATTGGGGAGAGG. However, the 3' primer does not match the proposed gene. These primers contain adaptor sequences. Template sequence provided by Paul Gray:
GNTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTCGCGGATCCTGTGAN
GCTCCTCTCAGCTGCTGTATGTGATCCAGGTCTAATTACAGGATACAAGGCAAAAACAGC
TCTGGGAGAACATTTGCTGAACGTTGGTGTGAATCGAGACAATGTGTCCGAGGTTTTGCA
GATTTTCATGGAGGCCCACTGTGGGCAGACTGAGCTGACTGAGAGCCTGAAGACTAAAGC
TTTCCAGGCTCACACTCCAGCACAGAAAGCGTCCATCCTGGCCTTCCTCGTTAACGAGCT
GGCATGCAGCAAGAGTGTGGTGAGTGAGATTGACAAGAACATTGAATACATGTCTAACTT
GAGAAGAGATAAATGGATGGTGGAAGGTAAACTCCGCAAGCTTAGGATAATTCATGCTAA
GAAAACAGGCAAGAGAGACGCCTCAGGGGGCATTGACCTCGGGGAAGAACAACACCCCTT
GGGCACGCCCACGCCAGGACGCAAGCGGAGAAGGAAGGGAGGGGACAGTGATTATGACGA
TGATGACGATGATGACAGCGACGACCAAGCTGATGAGGATGAGGAAGATGAGGAAGATAA
AGATGACAAAAAAGGAAAGAAGACCGATATCTGTGAAGATGAGGATGAAGGTGACCAAAC
AGCCAGCGTTGAGGAGCTAGAAAAACAGATTGAAAAACTGAGCAAACAACAGAGCCAGTA
CAGACGAAAGCTCTTTGATGCTTCTCACTCACTACGCTCGATGATGTTTNGGTCAGGATC
GGTACCGACGCAGATACTGGATTCTCCCTCAATGTGGANGGATTTTTGTAAAANGCATGG
AAAAGTGNTGAANGNCTANAAAAATTGCCAAAAAAAAAAAAAGCTTAAAANGGGGAGAGT
CTCCGATCAAGAANANTATTNGAACTCTGNGGNACNTTAACTGTTCCTTNNNATCCTGNG
GCGNAAACGACCCAAGAAAGAANCCAATNTTTCNTCAAANNGGCCTTTTCCAGTTAAANC
TTTAAGNACCAAAGCCCCGNGCNANNTNGNCCCCCNAGGGNGTTTC
Analysis Tools