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pM-gamma-1-Crl Probe Detail
Nucleotide
Probe/Clone
  • Name
    pM-gamma-1-Crl
  • Sequence Type
    cDNA
  • ID
    MGI:11936
  • Child Clone
    gamma-D probe
  • Vector Type
    Plasmid
  • Insert Site
    PstI
  • Insert Size
    463 bp
  • Note
    At low to medium stringency conditions this probe will cross hybridize to Crygf, b and a. This clone represents about 60% of full-length gamma crystallin mRNA.
  • Synonyms
    pM-gamma-1, pMgamma1
Source
  • Species
    mouse, laboratory
  • Strain
    NIH
  • Age
    postnatal day 5-10.0
  • Tissue
    lens
Genes
Crygd crystallin, gamma D
Sequences
K02583 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
Polymorphisms
J:9713 Skow LC, et al., Biochem Genet. 1988 Oct;26(9-10):557-70
Notes: Also C57BL/6J and C3H/HeJ differ using HincII - used to type RIs.
Endonuclease Gene Allele Fragments Strains
BamHI Crygd b 6.8,4.5,2.4,1.8kb C57BL/6J
d 9.5,4.5,1.8kb DBA/2J
HincII Crygd l 6.6,6.2,5.0,3.0,2.5,1.0, 0.5kb SJL/J
s 6.6,5.2,5.0,3.0,1.8,1.0, 0.5kb SWR/J
PstI Crygd b 5.0,4.8,2.8kb C57BL/6J
h 9.4,4.8,2.8kb C3H/HeJ
J:10663 Eicher EM, et al., Genetics. 1990 Jun;125(2):431-46
Endonuclease Gene Allele Fragments Strains
BamHI/BglI Cryg n 2.3kb NZB
s 2.7kb SM
References
J:6796 Shinohara T, et al., Multiple gamma-crystallins of the mouse lens: fractionation of mRNAs by cDNA cloning. Proc Natl Acad Sci U S A. 1982 May;79(9):2783-7
J:7698 Breitman ML, et al., Gamma-crystallin family of the mouse lens: structural and evolutionary relationships. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7762-6
J:9713 Skow LC, et al., Mapping of mouse gamma crystallin genes on chromosome 1. Biochem Genet. 1988 Oct;26(9-10):557-70
J:10663 Eicher EM, et al., The NXSM recombinant inbred strains of mice: genetic profile for 58 loci including the Mtv proviral loci. Genetics. 1990 Jun;125(2):431-46
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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