pH2.3 Probe Detail MGI Mouse MGI:11599

pH2.3 Probe Detail

Name

pH2.3
Sequence Type

genomic
ID

MGI:11599
Region Covered

includes the entire coding region
Vector Type

Plasmid
Insert Site

BamHI/HindIII
Insert Size

2.3kb
Other IDs

57494 (ATCC)

Species

human

Hspa2	heat shock protein 2
Hspa1b	heat shock protein 1B
Hspa8	heat shock protein 8

Assay Results

5

M11717 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)

M15432 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)

J:10825 Gaskins HR, et al., Immunogenetics. 1990;32(4):286-9

Notes: Using DraI, alleles a and d were indistinguishable and alleles b and c were indistinguishable. KpnI will distinguish between the NON/Lt and NOD/Lt strains.

Endonuclease	Gene	Allele	Fragments	Strains
DraI	Hspa1b	a	8.5kb	A/J, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, NON/ShiLt, NZB/BlNJ, NZW/LacJ, PL/J, RIIIS/J, SJL/J
		b	7.0kb	SM/J, SWR/J
		c	7.0kb	NOD/ShiLt
		d	8.5kb	M. spretus
EcoRI	Hspa1b	a	7.5kb	C57L/J, SJL/J
EcoRI	Hspa1b	b	6kb	SWR/J
SacI	Hspa1b	a	12,16kb	A/J, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, NON/ShiLt, NZB/BlNJ, NZW/LacJ, PL/J, RIIIS/J, SJL/J
		b	16,17kb	SM/J, SWR/J
		c	16kb	NOD/ShiLt
		d	8.4,19kb	M. spretus

J:18817 Khan F, et al., Genomics. 1994 May 1;21(1):128-37

Endonuclease	Gene	Allele	Fragments	Strains
EcoRI	Hspa2	a	6.6kb	AKR/J, DBA/2J, SM/J
EcoRI	Hspa2	b	5.5kb	BALB/cJ, C3H/HeJ, C57BL/6J, C57L/J, NZB/BlNJ, O20/A, SJL/J, SWR/J

J:15357 Hunt C, et al., Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6455-9

J:15358 Krawczyk Z, et al., A hsp70-related gene is constitutively highly expressed in testis of rat and mouse. Mol Biol Rep. 1987;12(1):27-34

J:10825 Gaskins HR, et al., Localization of a mouse heat shock Hsp70 gene within the H-2 complex. Immunogenetics. 1990;32(4):286-9

J:1314 Lafuse WP, et al., PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex. Immunogenetics. 1992;36(2):110-6

J:18817 Khan F, et al., Refinement of the DNA marker maps of mouse chromosome 12. Genomics. 1994 May 1;21(1):128-37

J:45236 Hatayama T, et al., Characteristic expression of high molecular mass heat shock protein HSP105 during mouse embryo development. Cell Struct Funct. 1997 Oct;22(5):517-25

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