Tg(Camk2a-MAPT*)784Hmor
Transgene Detail

Summary

• Symbol: Tg(Camk2a-MAPT*)784Hmor
• Name: transgene insertion 784, Hiroshi Mori
• MGI ID: MGI:8331900
• Synonyms: tau784
• Transgene: Tg(Camk2a-MAPT*)784Hmor Location: unknown
• Alliance: Tg(Camk2a-MAPT*)784Hmor page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H/He)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Camk2a-MAPT*)784Hmor expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MAPT (4137)

  Mapt	(MGI:97180)

  contains a C to T mutation at position +16 (intron 10 + 16C to T) relative to the 5' splice site in intron 10

• Mutation details: This transgene contains the mouse 8.6-kb calcium/calmodulin-dependent kinase II alpha promoter, the longest MAPT isoform, human tau441, cDNA with partial sequences of MAPT introns 9 and 10 inserted into the corresponding sides of exon 10 with a C to T mutation at position +16 (intron 10 + 16C to T) relative to the 5' splice site (predicted to lie within the stem) in intron 10 which increases splicing of exon 10. This line expresses tau protein levels of 186% of those in non-transgenic littermates, representing levels of human tau of approximately 86% of the levels of endogenous mouse tau. RT-PCR shows that mice exclusively express mRNA without exon 10 at 7 days of age and mRNA with and without exon 10 at a ratio of approximately 1:1 at 4 months of age. Quantification of signals of the alkaline phosphatase-treated samples show that mice predominately expresses 4R human tau (4R/3R ratio of 12.1) indicating altered splicing of exon 10 to increase 4R tau expression at an adult age. (J:197417)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:197417 Umeda T, et al., Neurodegenerative Disorder FTDP-17-Related Tau Intron 10 +16C-->T Mutation Increases Tau Exon 10 Splicing and Causes Tauopathy in Transgenic Mice. Am J Pathol. 2013 Jul;183(1):211-25
• All: 2 reference(s)