Tg(Slc32a1-tdTomato)54Rkan
Transgene Detail

Summary

• Symbol: Tg(Slc32a1-tdTomato)54Rkan
• Name: transgene insertion 54, Ryosuke Kaneko
• MGI ID: MGI:7524239
• Synonyms: Tg(Slc32a1-floxedSTOP-tdTomato)54Rkan, VGAT-stop-tdTomato Line #54
• Transgene: Tg(Slc32a1-tdTomato)54Rkan Location: unknown
• Alliance: Tg(Slc32a1-tdTomato)54Rkan page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Slc32a1-tdTomato)54Rkan involves 1 genes/genome features (Gm12442) View all
• Tg(Slc32a1-tdTomato)54Rkan expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc32a1 (MGI:1194488)

• Mutation details: The tdTomato cDNA with an inserted sequence of the intron between exon 1 and 2 of the mouse Slc32a1 gene containing a floxed stop sequence followed by a rabbit beta-globin polyadenylation (polyA) signal was inserted into the mouse Slc32a1 locus in a BAC clone (#160L22) using homologous recombination in Escherichia coli replacing the ATG codon plus 16 bp of the coding sequence in the Slc32a1 gene. This is a representative record representing five lines (Lines 28, 39, 47, 54, and 58) generated. Line 54 disrupted Gm12442 locus. Cre-mediated excision of the stop sequence is required for tdTomato expression. (J:257167)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:257167 Kaneko R, et al., Inhibitory neuron-specific Cre-dependent red fluorescent labeling using VGAT BAC-based transgenic mouse lines with identified transgene integration sites. J Comp Neurol. 2018 Feb 15;526(3):373-396
• All: 1 reference(s)