TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)72Nagy
Transgene Detail

Summary

• Symbol: TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)72Nagy
• Name: transgenic transposon concatemer 72, Andras Nagy
• MGI ID: MGI:6506983
• Synonyms: OKMSCh250
• Transgene: TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)72Nagy Location: unknown Genetic Position: Chr13, Syntenic
• Alliance: TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)72Nagy page

Transgene origin

• Strain of Origin: 129 and ICR

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter, Transposon concatemer)
• Mutation: Insertion
• TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)72Nagy involves 1 genes/genome features (A530065N20Rik) View all
• TgTn(pb-tetO-Pou5f1,-Klf4,-Myc,-Sox2,-mCherry)72Nagy expresses 4 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Klf4 (MGI:1342287)
  mouse	Myc (MGI:97250)
  mouse	Pou5f1 (MGI:101893)
  mouse	Sox2 (MGI:98364)

• Mutation details: The transgene consists of a piggy-BAC transposon delivery construct PB:TetO-OKMS-mCherry. PB:TetO-OKMS-mCherry contains a tetracycline responsive element (TRE; tetO), the polycistronic expression cassette OKMS, an IRES, the mCherry gene and polyA signal. The second generation TRE (SG-TRE) used here is tetO2 - containing a shortened CMV minimal promoter with eight copies of 19 bp tet operator sequences positioned in an optimized manner upstream of the TATA box. The OKMS cassette contains four mouse reprogramming genes - Oct4 (Pou5f1), Klf4, c-Myc (Myc) and Sox2 - each separated by viral 2A oligopeptides that mediate ribosomal skipping (F2A, E2A and F2A, respectively). Founder line 72 harbors a single transgene insertion into A530065N20Rik on chromosome 13:60,045,383-60,045,387[mm9] in transcriptionally reverse orientation. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)