Tg(Myh6/tetO-Sypl2)1Jmol
Transgene Detail

Summary

• Symbol: Tg(Myh6/tetO-Sypl2)1Jmol
• Name: transgene insertion 1, Jeffery Molkentin
• MGI ID: MGI:5897324
• Synonyms: Mg29 Tg
• Transgene: Tg(Myh6/tetO-Sypl2)1Jmol Location: unknown
• Alliance: Tg(Myh6/tetO-Sypl2)1Jmol page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Myh6/tetO-Sypl2)1Jmol expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sypl2 (MGI:1328311)

• Tg(Myh6/tetO-Sypl2)1Jmol expression driven by 2 genes
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myh6 (MGI:97255)
  Not Specified	tetO

• Mutation details: A cDNA encoding mouse Sypl2 (synaptophysin like 2; also called mitsugumin 29 or Mg29) is expressed under the regulatory control of the mouse Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter and the tetracycline responsive element (tetO). (J:252890)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Progeny of mice crossed with a Myh6-tTA strain are not viable unless doxycycline (DOX) is administered. Double transgenic animals not treated with DOX develop severe dilated cardiomyopathy and die between 4-8 weeks of age. In the presence of DOX, a greater than 50% loss of SYPL2 expression is seen in the heart. This lower expression of SYPL2 protein in the DOX-treated animals localizes to t-tubules adjacent to RYR2 (ryanodine receptor 2, cardiac) in the apposing SR.

References

• Original: J:252890 Correll RN, et al., Mitsugumin 29 regulates t-tubule architecture in the failing heart. Sci Rep. 2017 Jul 13;7(1):5328
• All: 1 reference(s)