Tg(OPTN*E50K,-EGFP)1Mde
Transgene Detail

Summary

• Symbol: Tg(OPTN*E50K,-EGFP)1Mde
• Name: transgene insertion 1, Michael D Ehlers
• MGI ID: MGI:5705748
• Synonyms: BAC-hOPTN^E50K
• Transgene: Tg(OPTN*E50K,-EGFP)1Mde Location: unknown
• Alliance: Tg(OPTN*E50K,-EGFP)1Mde page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(OPTN*E50K,-EGFP)1Mde expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  OPTN (10133)

  Optn	(MGI:1918898)

• Mutation details: The transgene is composed of BAC clone RP11-1107F3 containing the 38 kb-human OPTN locus with about 160 kb of 5 sequence containing the E50K mutation in the middle (GAG to AAG) and a fragment containing the internal ribosome entry site-enhanced green fluorescent protein. Two lines were established, 1 and 2. Line 1 shows overexpression of the gene but line 2 does not. Quantitative rtPCR and immunoblot analysis confirmed mRNA expression and protein expression in the retina. (J:222642)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:222642 Tseng HC, et al., Visual impairment in an optineurin mouse model of primary open-angle glaucoma. Neurobiol Aging. 2015 Jun;36(6):2201-12
• All: 1 reference(s)