Tg(Ins1-cre)25Utr
Transgene Detail

Summary

• Symbol: Tg(Ins1-cre)25Utr
• Name: transgene insertion 25, Ken-ichi Yagami
• MGI ID: MGI:5560759
• Synonyms: BAC Ins1-cre25
• Transgene: Tg(Ins1-cre)25Utr Location: unknown Genetic Position: Chr15, Syntenic
• Alliance: Tg(Ins1-cre)25Utr page

Transgene origin

• Strain of Origin: C57BL/6N

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Ins1-cre)25Utr expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ins1 (MGI:96572)

• Mutation details: The targeting vector contained homology arms flanking a nuclear localization signal (NLS) fused to a fragment encoding cre recombinase with a polyadenylation signal. Red/ET recombination in bacterial cells inserted the NLS-cre sequence at the translation initiation site in exon 2 of the Ins1 gene located in a BAC (RP23-181I21). The engineered BAC was linearized and injected into pronuclei of fertilized C57BL/6N oocytes. Five founders had the inserted transgnene (#'s 7, 24, 25, 28 and 32). Line 25 was used further as recombinase reporter activity in the pancreas in F1 crosses was greater than that from other founders. (J:205139, J:208032)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ins1 (mouse) Summary of all recombinase alleles driven by Ins1. Comparative matrix view of recombinase activities Alliance mouse Ins1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:208032 Hasegawa Y, et al., Generation and Characterization of Ins1-cre-driver C57BL/6N for Exclusive Pancreatic Beta Cell-specific Cre-loxP Recombination. Exp Anim. 2014;63(2):183-91
• All: 7 reference(s)