Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  17
• Reference
  J:10895 Barlow DP, et al., The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus. Nature. 1991 Jan 3;349(6304):84-7
• ID
  MGI:47933

Genes

Gene	Assay Type
Map3k4	PFGE
Plg	PFGE
Igf2r	PFGE
Tme	reported elsewhere
Tcp1	PFGE
Sod2	PFGE
D17Leh66Db1	PFGE
D17Leh66Db2	PFGE
D17Leh66Dg1	PFGE
D17Leh66Dg2	PFGE
D17Leh66Dg3	PFGE

Notes

• Reference
  Authors use D17Leh66D which has been withdrawn to equal D17Leh66Db1, D17Leh66Db2, D17Leh66Dg1, D17Leh66Dg2 and D17Leh66Dg3.
• Experiment
  Southern analysis using various probes was informative in defining the region of t^Lub2 containing Tme as being flanked by markers Plg and Sod2, to further refine this region, physical mapping using pulsed-field gels of BALB/c genomic DNA was employed.The maximum distance separating Plg and Sod2 was indicated as 1.1 Mb. The minimum distance separating Plg and Sod2 is 0.8 Mb. Using NotI, MluI, BsshIII and NruI digests the following order of loci in approximately 3.8kb of genomic DNA was determined to be as follows: D17Rp17E - Plg - Igf2r - Tcp1 - Sod2 - D17Leh66Db1 - D17Leh66Db2 - D17Leh66Dg1 - D17Leh66Dg2 - D17Leh66Dg3 again with Tme mapping between Plg and Sod2.