Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  10
• Reference
  J:34619 Kapfhamer D, et al., The neurological mouse mutations jittery and hesitant are allelic and map to the region of mouse chromosome 10 homologous to 19p13.3. Genomics. 1996 Aug 1;35(3):533-8
• ID
  MGI:47886

Genes

Gene	Assay Type	Description
D10Mit158	PCR amplified length variant
Pfkl	reported elsewhere
D10Mit175	PCR amplified length variant
D10Mit207	PCR amplified length variant
Bsg	reported elsewhere
Atcay	visible phenotype
Amh	reported elsewhere
D10Mit7	PCR amplified length variant
D10Mit23	PCR amplified length variant
D10Mit42	PCR amplified length variant
D10Mit140	PCR amplified length variant
Nfyb	Southern analysis	YB-EM38
D10Mit160	PCR amplified length variant

Notes

• Reference
  Heterozygotes for hesitant (C3H-hes/+) and jittery (JIGR/Dn- ji +/+ gr) were mated with each other. Of 32 offspring, 12 clearly had the hesitant phenotype. This result established non-complementation of the two mutations. Cross mapping data which indicated co-localization of hes and ji on mouse Chromosome 10 established that hesitant and jittery are caused by mutant alleles of the same gene.
• Experiment
  In intersubspecific backcross of JIGR/Dn-gr +/+ ji mice with CASA/Rk was carried out. Initially, only F1 animals carrying a ji/+ genotype (as determined by a test cross) were typed, but in later stages of the cross, unaffected animals were also scored. The majority of offspring was derived from the (JIGR/Dn-gr +/Ji x CASA/Rk)F1 x JIGR/Dn-ji +/+ gr cross.Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, sexes unspecified or combined
• Female Parent
  <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j>/<c> <c> <c> <c> <c> <c> <c> <c> <c> <c> <c> <c> <c>
• Strain
  (JIGR/Dn Mfsd12 +/+ Atcay x CASA/Rk)F1-Atcay
• Male Parent
  <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j>/<j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j> <j>
• Strain
  JIGR/DnJ
• Allele 1
  j from JIGR/DnJ
• Allele 2
  c from JIGR/DnJ

CROSS Data

MC #mice	D10Mit158	Pfkl	D10Mit175	D10Mit207	Bsg	Atcay	Amh	D10Mit7	D10Mit23	D10Mit42	D10Mit140	Nfyb	D10Mit160
30	c	.	.	.	.	c	c	c	c	.	.	.	c
166	j	.	.	.	.	j	j	j	j	.	.	.	j
1	c	j	j	j	j	j	j	j	j	j	j	j	j
1	j	c	c	c	c	c	c	c	c	c	c	c	c
1	c	c	j	j	j	j	j	j	j	j	j	j	j
0	j	j	c	c	c	c	c	c	c	c	c	c	c
1	j	j	j	j	j	j	j	j	j	j	j	j	c
0	c	c	c	c	c	c	c	c	c	c	c	c	j

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
Atcay	Amh	0	200	0.000	0.000
Amh	D10Mit7	0	200	0.000	0.000
D10Mit7	D10Mit23	0	200	0.000	0.000