Mapping Data

Experiment

• Experiment
  TEXT-Cytogenetic Localization
• Chromosome
  16
• Reference
  J:29232 Reeves RH, et al., A mouse model for Down syndrome exhibits learning and behaviour deficits [see comments]. Nat Genet. 1995 Oct;11(2):177-84
• ID
  MGI:45659

Genes

Gene	Assay Type	Description
Pgk1-ps1	Southern analysis	B24
D16Mit68	PCR amplified length variant	MPC429
T(16C3-4;17A2)65Dn
App	Southern analysis	pDS10.1

Notes

• Experiment
  Five mouse x Chinese hamster somatic cell hybrid lines were used to more finely map the T(16;17)65Dn translocation breakpoint. Hybrid lines H65B3.2a-2, -8, -13, and -14 carry Chromosome 16 material proximal to the translocation breakpoint, and hybrid line H65B3.2a-1 carries Chromosome 16 material distal to the breakpoint. The hybrid lines carrying the proximal chromsome material were all positive for the marker Pgk1-ps1 and negative for App, while the one hybrid carrying distal chromosome material was negative for Pgk1-ps1 and App. All 5 hybrid lines were negative for D16Mit68, which lies between Pgk1-ps1 and App. This leads the authors to conclude a small portion of Chromsome 16 may have been deleted from the chromosome at the time of the translocation.Additionally, this places the T(16;17)65Dn translocation breakpoint at the D16Mit68 locus.