Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  3
• Reference
  J:25562 Reiner O, et al., Lissencephaly gene (LIS1) expression in the CNS suggests a role in neuronal migration. J Neurosci. 1995 May;15(5 Pt 2):3730-8
• ID
  MGI:45219

Genes

Gene	Assay Type	Description
Tpi-rs2	Southern analysis	pHTPI-5A
D3Mit22	PCR amplified length variant	D122
Pafah1b1-ps1	Southern analysis	LIS-ORF
Ngf	Southern analysis	pmngf6

Notes

• Reference
  Authors use Lis1, Lis3, Lis4 and Evi2a for the locus symbols Mdsh, Mdsh3, Mdsh4 and Evi2 respectively.
• Experiment
  Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, female
• Female Parent
  <a> <a> <a> <a>/<s> <s> <s> <s>
• Strain
  (AEJ/Gn-a Gdf5/a Gdf5 x M. spretus)F1
• Male Parent
  <a> <a> <a> <a>/<a> <a> <a> <a>
• Strain
  AEJ/Gn-a Gdf5
• Allele 1
  a from AEJ/Gn-a Gdf5
• Allele 2
  s from M. spretus

CROSS Data

MC #mice	Tpi-rs2	D3Mit22	Pafah1b1-ps1	Ngf
30	a	a	a	a
42	s	s	s	s
1	a	s	s	s
4	s	a	a	a
4	a	a	s	s
3	s	s	a	a
2	a	a	a	s
3	s	s	s	a

Additional Mice Typed Only for Specific Pairs of Loci

Marker 1	Marker 2	# Recombinants	# Parentals
Tpi-rs2	D3Mit22	0	3
Pafah1b1-ps1	Ngf	7	54

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
Tpi-rs2	D3Mit22	5	92	5.435	2.364
D3Mit22	Pafah1b1-ps1	7	89	7.865	2.853
Pafah1b1-ps1	Ngf	12	150	8.000	2.215