Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  12
• Reference
  J:15634 D'Eustachio P, et al., Localization of the twitcher (twi) mutation on mouse chromosome 12. Mamm Genome. 1993 Nov;4(11):684-6
• ID
  MGI:42573

Genes

Gene	Allele	Assay Type	Description
D12Nyu2		Southern analysis	pUCO19-25
D12Mit2		PCR amplified length variant	M27
Sptb		Southern analysis	Spnb1 probe
D12Mit5		PCR amplified length variant	L58
Fos		Southern analysis	Fos probe
Mtv9		Southern analysis	pMtv9-5'c
Galc	Galc^twi	visible phenotype
D12Mit7		PCR amplified length variant	M62
Serpina1		Southern analysis	pC1.2
Igh-V3609		Southern analysis

Notes

• Experiment
  Genotypes of 60 twi/twi intercross progeny were provided. The deduced haplotypes are presented here. Number of chromosomes typing ddbbb was reported as 13 but changed to 14 by Dr. Peter D'Eustachio. The data presented in the 2x2 table includes the combined linkage data from crosses between laboratory strains or between laboratory strains and Mus castaneus. These crosses are reported in Birkenmeier, C.S., et al., PNAS USA 85:8121-8125, 1988: Blank, R.D. et al., Genetics 120:1073-1093, 1988: de Meeus, A., et al., Mammalian Genome 3:637-643, 1992: Dietrich, W. et al., Genetics 131:423-47, 1992: and D'Eustachio, P. Mammalian Genome 4, Special Issue S176-S191, 1993. Numbers represent chromosomes tested.

CROSS

• Type
  Intercross, treated like backcross(i.e. # chromosomes scored)
• Female Parent
  <b> <b> <b> <b> <b> <b> <b> <b> <b> <b>/<d> <d> <d> <d> <d> <d> <d> <d> <d> <d>
• Strain
  (C57BL/6J x DBA/2J)F1-Galc
• Male Parent
  <b> <b> <b> <b> <b> <b> <b> <b> <b> <b>/<d> <d> <d> <d> <d> <d> <d> <d> <d> <d>
• Strain
  (C57BL/6J x DBA/2J)F1-Galc
• Allele 1
  b from C57BL/6J
• Allele 2
  d from DBA/2J

CROSS Data

MC #mice	D12Mit2	D12Mit5	Galc	D12Mit7	Serpina1
78	b	b	b	b	b
22	d	b	b	b	b
14	d	d	b	b	b
4	b	b	b	d	d
1	b	b	b	b	d
1	d	d	b	d	d

Additional Mice Typed Only for Specific Pairs of Loci

Marker 1	Marker 2	# Recombinants	# Parentals
D12Nyu2	D12Mit2	9	105
D12Nyu2	Sptb	36	117
D12Nyu2	D12Mit5	26	88
D12Nyu2	Fos	86	231
D12Nyu2	Mtv9	117	280
D12Nyu2	Sptb	42	75
D12Nyu2	D12Mit7	44	70
D12Nyu2	Serpina1	163	268
D12Mit2	D12Nyu2	9	105
D12Mit2	D12Mit7	35	175
D12Nyu2	Fos	20	60
D12Nyu2	D12Mit5	25	83
Sptb	Fos	12	213
Sptb	Fos	14	139
Sptb	Fos	34	191
D12Mit2	D12Nyu2	26	88
D12Mit2	Fos	5	75
D12Mit2	D12Mit5	22	68
Fos	Fos	10	316
Fos	Fos	44	345
Fos	D12Nyu2	21	59
Fos	D12Nyu2	20	60
Fos	D12Mit2	5	75
Fos	Sptb	5	75
Fos	D12Mit5	7	73
Fos	Mtv9	34	363
Sptb	D12Nyu2	42	72
Sptb	Fos	5	75
D12Mit5	D12Nyu2	44	70
D12Mit5	Fos	7	73
D12Mit5	Fos	6	109
Fos	D12Nyu2	45	69
Fos	Mtv9	8	72
Igh-V3609	D12Nyu2	180	187
Igh-V3609	D12Mit2	58	144
Igh-V3609	Sptb	51	102
Igh-V3609	D12Mit5	45	99
Igh-V3609	Fos	90	227
Igh-V3609	Mtv9	93	270
Igh-V3609	D12Mit7	21	121
Igh-V3609	Serpina1	63	310