Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:93219 Everett CA, et al., Genetic influences on ovulation of primary oocytes in LT/Sv strain mice. Reproduction. 2004 11;128(5):565-571
• ID
  MGI:3511870

Genes

Gene	Assay Type
Popoa	visible phenotype
D1Mit306	PCR amplified length variant
D1Mit382	PCR amplified length variant
D1Mit498	PCR amplified length variant
Map2	reported elsewhere
Kif22-ps	reported elsewhere
Kif21b	reported elsewhere
Ncl	reported elsewhere
Serpine2	reported elsewhere
Bcl2	reported elsewhere
Psmb6-ps2	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 124 (CAST/Ei x LT/SvKau)F1 x LT/SvKau backcross animals to identify QTLs associated with premature ovulation and oocyte arrest. Parental strain LT/SvKau exhibits a high proportion of diploid primary oocyte ovulation and oocyte arrest in meiotic metaphase I. This phenotype shows incomplete penetrance and variable expressivity. 59 polymorphic markers were used in the initial genome scan. Linkage to the phenotype was detected on mouse Chromosomes 1, 9, and 17. An additional37 polymorphic markers were used to refine these loci.
  
  The strongest linkage mapped to 58.7 cM on mouse Chromosome 1 with LOD=5 at D1Mit306. This locus is named Popoa (premature ovulation and primary oocyte arrest). The QTL interval of Popoa spans approximately 50 cM (D1Mit382) - 73 cM (D1Mit498). LT/SvKau-derived alleles confer increased metaphase I oocyte arrest at Popoa. Several candidate genes (Mtap2, Kif22-ps, Kif21b, Ncl, Serpine2, Bcl2, Psmb6-rs1) map near Popoa but sequence analysis did not reveal any polymorphisms between CAST/Ei and LT/SvKau.
  
  Suggestive linkage to metaphase I arrest mapped to 48 cM on mouse Chromosome 9 with LOD=2.5 at D9Mit181. Several candidate genes map to this region: Map2k1, Omt2a, Omt2b, Bcl2a1a, Mtap4, and Cdc25a. Sequence analysis did not reveal any polymorphisms between CAST/Ei and LT/SvKau.
  Oriq1 (31 cM) is a previously identified ovulation rate QTL mapping near this locus.
  
  A locus on mouse Chromosome 17 was detected near D17Mit209 in the initial genome scan but didnot produce a significant LOD score after typing of additional markers.