Experiment
A (C57BL/6ByJ x 129P3/J)F1 x (C57BL/6ByJ x 129P3/J)F1 intercross was used to map the following markers on mouse Chromosome 4 around the Sac locus. 280G12-T7 - 0.1 cM - 49O2-T7 - 118E21-SP6 - 0.1 cM - M134G01 - 0.1 cM - Tas1r3 - 0.1 cM - 350D2-T7 - 0.3 cM - 139J18-SP6 - D4Mon1 - 0.1 cM - 280G12-SP6 . Taslr3 and Sac are equivalent. The Authors provided the following mapping information to MGI: Total number of chromosomes typed: 1258 (629 F2 mice). DNA mapping panel used: new panel, an F2 intercross between 129P3/J and C57BL/6ByJ strains. The animals were produced from reciprocal outcrossing of 129P3/J females and C57BL/6ByJ males, or C57BL/6ByJ females and 129P3/J males. Centromere - D4Mit33 - 2.1 cM - D4Mit190 - 1.0 cM - D4Mit42- 0.2 cM - Tas1r1 (aliasGpr70)- 0.9 cM - D4Mit254 - 0.1 cM - Trp73 - 0.6 cM - D4Mt209 - 2.8 cM - D4Mit256 - 0.2 cM - D4Smh6b - 0.7 cM - [360M12-SP6, 415A22-SP6] - 1.2 cM - 280G12-T7 - 0.1 cM - [49O2-T7, 118E21-SP6, 387F5-SP6, 338N4-T7] - 0.1 cM - M134G01 - 0.1 cM - [Tas1r3 (alias Sac), D18346] - 0.1 cM - 350D2-T7 - 0.3 cM - D4Mon1 - 0.1 cM - [130A12-SP6, 338N4-SP6, 387F5-T7, 280G12-SP6] - 0.8 cM - 139J18-T7 - 1.4 cM - K00231 - 0.1 cM - 238O5-T7 - 0.4 cM - [v2r2, D4Ertd296e] - Telomere. Interval D4Mit33- [v2r2, D4Ertd296e] is 13.3 cM. Markers shown within brackets could not be ordered. Genetic distances between markers were estimated using MAPMAKER/EXP and shown in Fig. 1a of the manuscript.
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