Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  5
• Reference
  J:50926 Hough RB, et al., Rump white inversion in the mouse disrupts dipeptidyl aminopeptidase-like protein 6 and causes dysregulation of Kit expression. Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13800-5
• ID
  MGI:1321227

Genes

Gene	Assay Type
D5Buc2	overlapping clones, direct sequencing, PFGE
Pdgfra	overlapping clones, direct sequencing, PFGE
D5Buc3	overlapping clones, direct sequencing, PFGE
D5Buc4	overlapping clones, direct sequencing, PFGE
D5Buc5	overlapping clones, direct sequencing, PFGE
Kit	overlapping clones, direct sequencing, PFGE
Rw	overlapping clones, direct sequencing, PFGE, visible phenotype
Ph	overlapping clones, direct sequencing, PFGE, visible phenotype
En2

Notes

• Experiment
  The authors used overlapping clones, direct sequencing, and PFGE to construct a physical map of the Rw and Ph mutations on mouse Chromosome 5. Rw mice were found to contain an inversion with the breakpoint of the inversion located within the Dpp6 gene. The order of markers in a normal mouse was found to be D5Buc2 - Pdgfra - D5Buc3 - D5Buc4 - D5Buc5 - Kit. The Pdgfra, D5Buc3 and D5Buc4 markers were missing in the Ph deletion. Based on PFGE analysis D5Buc5 is located 160-220 bps from Kit.