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| Caption | Generation of the Cdkn1atm2Hpw allele. (A) Structure of the targeting vector, wild-type Cdkn1a (p21) genomic allele (+), and recombinant allele before (f) and after (L) Cre-mediated excision. The coding region of the mouse Cdkn1a gene was disrupted by insertion of a neomycin phosphotransferase cDNA cassette followed by a modified stop cassette (Neo-STOP) with each of the 3 stop codons in each reading frame (to reduce any read-through driven by the phosphoglycerine kinase promoter), followed by a codon-optimized click-beetle red luciferase cDNA (Luc) and bovine growth hormone polyadenylation sequences. Upon homologous recombination, the Luc cDNA utilizes the ATG start codon of endogenous Cdkn1a in exon 2. Exons are represented by black boxes; 5' and 3' arms are highlighted in teal; Sph I sites are denoted by S; Nde I sites are denoted by N; Bgl II sites are denoted by Bg; loxp sites are shown by blue triangles; the 5' Southern probe is shown by a gray box; the 3' Southern probe is shown by a red box; PCR primers are shown by black arrows and lowercase letters. | ||||
| Copyright | This image is from Tinkum KL, J Biol Chem 2013 Sep 27;288(39):27999-8008 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:199432 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 01/06/2026 MGI 6.24 |
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