Cdkn1a^tm2Hpw
Targeted Allele Detail

Summary

• Symbol: Cdkn1a^tm2Hpw
• Name: cyclin dependent kinase inhibitor 1A; targeted mutation 2, Helen Piwnica-Worms
• MGI ID: MGI:5502584
• Synonyms: p21-CBRLuc, p21^f
• Gene: Cdkn1a Location: Chr17:29309953-29319696 bp, + strand Genetic Position: Chr17, 15.12 cM
• Alliance: Cdkn1a^tm2Hpw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199432
• Parent Cell Line: SCC10 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: A loxP-flanked neomycin/stop cassette was introduced to exon 2, 5' of the ATG start codon by homologous recombination. Codon-optimized click-beetle red luciferase cDNA (CBRLuc) and bovine growth hormone polyadenylation sequences were added just 3' of the start codon. (J:199432)

Generation of the Cdkn1a^tm2Hpw allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdkn1a Mutation: 63 strains or lines available

References

• Original: J:199432 Tinkum KL, et al., Forkhead box O1 (FOXO1) protein, but not p53, contributes to robust induction of p21 expression in fasted mice. J Biol Chem. 2013 Sep 27;288(39):27999-8008
• All: 1 reference(s)