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| Caption | Generation of the Plcg1tm1Rwen allele. The diphtheria toxin A (DTA) cDNA driven by the thymidine kinase promoter was cloned 5' of the targeting vector. The first loxP site was inserted into intron 1 along with SstII site. The neo cassette was inserted into intron 4 immediately followed by the second loxP site. Transgenic expression of Cre recombinase can delete exons 2, 3, and 4 converting the floxed Plcg1tm1Rwen allele to a null allele. The boxes represent exons. The floxed sites are depicted by closed arrowheads. The location of the 3' external probe and PCR primers (P1 to P4) for screening embryonic stem cells containing the appropriate homologous recombination were indicated. Southern blot analysis of XbaI-digested genomic DNA with the 3' external probe could detect a 13-kb WT allele fragment and a 9.5-kb homologous recombinant allele fragment. Primers P1 and P2 could detect a 3.1-kb WT allele fragment, primers P1 and P3 could detect a 4.0-kb homologous recombinant allele fragment, and primers P1 and P4 could detect a 2.1-kb allele fragment with exons 2 to 4 deleted. The construct was electroporated into 129/SvEv ES cells, and the positive clones with normal karyotypes were then microinjected into blastocysts at inGenious Targeting Laboratory. | ||||
| Copyright | This image is from Fu G, J Exp Med 2010 Feb 15;207(2):309-18, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:157757 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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